Znrf1 (NM_133206) Mouse Untagged Clone

CAT#: MC200768

Znrf1 (untagged) - Mouse zinc and ring finger 1 (Znrf1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Znrf1
• Synonyms: B830022L21Rik; nin283; Rnf42; Zrfp1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC006765 sequence for NM_133206
  CAACCCTCCGCCTTTATGTTCGCCCAGACCTCCACCCGCTTCTGAGTAGTGGGGGAGGGTTTCAGCCTCC ACGTTCCCGCCCCACCGGGGCCCCGGCGAACATGGGGGGCAAGCAGAGCACGGCGGCCCGCTCTCGGGGC CCCTTCCCGGGGGTCTCTACGGATGACAGCGCCGTGCCGCCGCCGGGAGGGGCGCCCCACTTTGGGCACT ACCGGACGGGCGGCGGGGCGATGGGGCTGCGCAGCCGCTCGGTCAGCTCGGTGGCGGGCATGGGCATGGA CCCCAGCACGGCCGGAGGGGTGCCCTTTAGTCTCTACACCCCCGCCTCCCGCGGGACCGGCGACTCCGAG AGGGCGCCGGGCGGCGGAGGGTCCACGTCGGACTCCACCTATGCCCACGGCAATGGTTACCAAGAGACCG GCGGCGGTCACCATAGAGACGGGATGCTGTACCTGGGCTCCCGAGCCTCGCTGGCGGATGCTCTACCTCT GCACATCGCACCCAGGTGGTTCAGCTCGCACAGTGGTTTCAAGTGCCCCATTTGTTCCAAGTCTGTGGCT TCCGATGAGATGGAAATGCACTTTATAATGTGTCTGAGCAAGCCTCGCCTGTCCTACAATGATGATGTGC TGACTAAAGATGCGGGTGAGTGTGTGATCTGCCTGGAGGAGCTGCTTCAGGGGGACACGATAGCCAGGCT GCCTTGCCTGTGCATCTATCACAAAAGCTGCATAGACTCATGGTTTGAAGTGAACAGATCTTGTCCAGAG CACCCTGCTGACTGACCCTGCTGGGCCTGCTTGCCGACTCCTCTCAAAGGTGAGCCCATACTTGGCGGGA GACTCTGGGCTAAGAGTCAAGTTTCTGGGAGCTGGGGGAGAGGAGCTGGCCTGCCTGATACACACCCCAC CAAGGAGAAAGCAGAAGCAGAAAGACAGAAGCCATCTCCTCAGAGAGCAGTCCCCAGGAAGTAGGGGGAT GTTCCTGGGAGTTGGGGGAGCATGAAAGAGCGATAGTAACCTAGTCATGAGAACTCCATGCAAAGATAGA ACCAGGCTCCTTCCCCTCAGGAGAGGCAGAAGGTCCCCTGCAGGGTGCATGGGCTGGAGCAGAGGCGCCC CCAGCCAGGCTCTACAGCAGCCTCACCTCCCTCACCTCTCAGAGACTCGGTTCTGAAAGGGAAGAATCAC TAAGTAGTGGCCTGTGGAACAGACATTGTCCACTGCAAAGACATGGCCTGAGGTCTTTCCACTCCTGGTT CTTCCACCGGCCTGAGAGGTCCAGGCCACACTCAGTCCTTGTGTCCCAGGCACAGTGTCACAGCAGCAGG CTACTCCCTCCCACACACGCACTTCTGGTGCTGTGTAACCATTGGATCTGACCTTCATTTCTTCCCCAGG GTACCTTGTTTAACTGCCCCTATCCCGCCCCCACCCTGCCTGTGCCTTTGAGGACTGTGTTGGGTGTTCT CTCTCTTTTGTTTTGTTTAGTTTGTTTTTGTCCCATCCAACTGAAAACCATCAGCCCCTTCCCACTTGAT CCCACATCCAAAGGCTTGGGGATCCCTTTTCTTCTCTCTCTTCTTTGTTGTTTTTTCTTTTGTTAGGTTT ATTTTATTTATTCCAAGTAAATGCCCAGAACAGCTGCTGGTGTCAGGCAAATCAGGGACATTGGCTCTTT CTACCCTCACCTCAAAACCTGATGCCAAGCGAGGGAGAGTGAGGCAGAGGGACTGGATCCCAGCTCCAGA GTGCAGGTCCAGAATGCCGCCGCCGGCATGATGTTTGGCTTCCAATGGGCCTGCCTGGACTGAACCAGAT GCCCCAGCCAACCAGCCTGGTCTCAGGGGGACACAAGCTGCTGCCACCGTGCGATGGAGCGGCAACTCTC AGCAAGATGAAGCGGGACTGACTTCTTCCGACCCACACAGGGACAGCCAGCCCCTGTTCCTGGGAGGAGG CTCCTCGGACACTGGACAGAGCTGAGCTTGGGACACCAGAGAGAACAGGGCACCCTTCTGCACTGACTTC CAGAAAATGGTCCTCCCTGAGGACACCCAGTGGATGAGAGAAGAATGAATTGACCTCTATCCTTCCCCTC ACCCTCGACCCAGGAGGGAAAGGGCATTTTCTTTTTCACCTTTGAAAGGCGTTGTGGGTCTGTCTTTAAA AAGTGTTTACAAACAACAAAAAATTCTATAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_133206
• Insert Size: 684 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006765, AAH06765
• RefSeq Size: 2214 bp
• RefSeq ORF: 684 bp
• Locus ID: 170737
• UniProt ID: Q91V17
• Cytogenetics: 8 E1
• Gene Summary: E3 ubiquitin-protein ligase that mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL. Promotes Wallerian degeneration, a neurodegeneration disorder, by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons: degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1, 2, and 5 all encode the same isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.