Cenpb (BC006628) Mouse Untagged Clone

CAT#: MC200749

Cenpb (untagged) - Mouse centromere protein B (cDNA clone MGC:11737 IMAGE:3968847), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cenpb
• Synonyms: CENP-B
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC006628
  GGCCCCGGGGCGGAGGGCGCGCCGGGATGGGCCCCAAGCGGCGGCAGCTGACGTTCCGGGAGAAGTCGCG GATCATCCAGGAGGTGGAGGAGAACCCGGACCTACGCAAGGGCGAGATCGCGCGGCGCTTCAACATCCCG CCGTCCACGCTGAGCACCATCCTGAAGAACAAGCGCGCCATCCTGGCGTCGGAGCGCAAGTACGGAGTGG CCTCCACCTGCCGCAAGACCAACAAGCTGTCCCCGTACGACAAGCTGGAGGGGCTTCTCATCGCTTGGTT CCAGCAGATCCGCGCCGCCGGGCTGCCGGTCAAGGGCATCATCCTGAAAGAGAAGGCGCTGCGGATAGCG GAGGAGCTGGGCATGGACGACTTCACTGCTTCCAACGGCTGGCTGGATCGCTTCCGCAGGCGCCACGGTG TAGTGGCCTGCAGCGGCGTGACCCGTTCTCGGGCGCGAAGCTCTGCCCCTCGGGCTCCAGCGGCACCTGC CGGCCCAGCCACGGTGCCCTCCGAGGGCAGCGGTGGGAGCACACCCGGCTGGCACACGAGGGAGGAGCAG CCGCCGTCGGTAGCTGAGGGCTACGCCTCGCAGGACGTGTTCAGCGCCACCGAGACCAGCCTGTGGTACG ACTTTCTGTCGGACCAGGCCTCAGGGCTCTGGGGAGGTGATGGACCGGCGCGCCAGGCCACCCAGCGCCT TAGCGTTTTGCTGTGCGCCAACGCCGATGGCAGCGAGAAGCTTCCCCCACTGGTCGCAGGCAAATCTGCC AAGCCCCGTGCAGGGCAAGGTGGTCTGCCCTGCGACTACACTGCCAACTCGAAGGGTGGAGTCACCACCC AGGCTCTGGCTAAGTACTTGAAAGCTCTGGACACTCGAATGGCAGCAGAATCTCGTCGGGTCCTTCTGCT GGCCGGCCGTCTGGCTGCCCAGTCCTTGGACACCTCGGGCCTGCGGCACGTGCAGCTGGCCTTCTTCCCT CCTGGCACCGTGCATCCTTTGGAGCGAGGAGTGGTCCAACAGGTGAAGGGCCACTACCGCCAGGCGATGT TGCTCAAGGCCATGGCAGCACTCGAGGGCCAGGATCCCTCAGGCTTGCAACTGGGCCTTGTGGAGGCCCT GCACTTTGTGGCTGCAGCCTGGCAGGCAGTGGAGCCCTCGGACATAGCAACTTGCTTTCGTGAGGCGGGT TTTGGAGGTGGCCTTAATGCCACTATCACCACTTCCTTCAAGAGTGAGGGAGAGGAAGAGGAGGAAGAGG AGGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGGTGAAGGGGAAGAGGAGGAGGAAGAGGAAGAAGAAGG GGAGGAGGAAGGAGGGGAAGGAGAGGAGGAAGGAGAGGAAGAGGTAGAAGAGGAAGGTGAGGTTGATGAT AGTGATGAAGAGGAGGAAGAAAGTTCCTCTGAGGGTTTAGAGGCTGAAGACTGGGCCCAGGGAGTAGTGG AAGCCAGTGGTGGTTTTGGGGGTTATAGTGTCCAGGAAGAGGCCCAGTTCCCCACTCTCCATTTCCTGGA AGGAGGAGAGGACTCTGACTCAGACAGTGATGAAGAGGAGGACGATGAAGAGGAGGATGAGGAGGATGAA GATGAGGAGGATGATGAGGATGGTGATGAGGTCCCTGTGCCCAGCTTTGGGGAGGCCATGGCTTACTTTG CCATGGTCAAGAGGTACCTGACCTCCTTCCCCATCGATGACCGAGTGCAGAGTCACATCCTTCATTTGGA ACACGATCTGGTCCACGTGACTAGGAAGAACCGAGCCTGCCTCTGCCTGCTTTCCACCTCCCCAGATCCC TTTCTCTGGCCCAGTCTTTGGCCCTTGGTTTTCTTTCCAGATTGGAGGTTTCCAGGAGGCCCTCCAGGGG CGTGACAGCAGGCACCTCCAATCTGGGTAGCTACAGGCCTCTGCCTCATCCTCTGCAGACCCCCATCCTG GGCAGAGGGGCCTGGGGCTGGGCCCAGAGTCCAGCCACCCAGGTGCTCCTTTCCCAGCTTGAATTCAATA AATCTGTCCACTCCCCTTTTGTGGGAGTGAATGTTTTAACAGCCAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC006628
• Insert Size: 1869 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006628, AAH06628
• RefSeq Size: 2090 bp
• RefSeq ORF: 1869 bp
• Locus ID: 12616
• Cytogenetics: 2 63.29 cM
• Gene Summary: Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.[UniProtKB/Swiss-Prot Function]