Racgap1 (BC010715) Mouse Untagged Clone

CAT#: MC200700

Racgap1 (untagged) - Mouse Rac GTPase-activating protein 1 (cDNA clone MGC:11396 IMAGE:3602242), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Racgap1
• Synonyms: Band25, GTPase, MgcRacGAP, gtl11
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC010715
  GTCGCTGGTAGACAGCAGCCCTCCTGGGGCGCGGCGGCCGGAGAGTGAGCGCCGCGGGCCGGACGGGGTT GCGTGTGCGCGGGGCCGGGAGCCTCGAAAGATGGATACTACAATGGTGAATTTGTGGACTCTGTTTGAGC AGCTTGTGCGCCGGATGGAGATTATCAATGAAGGAAATGAAAGCATTGAATTCATCCAGGTTGTGAAGGA CTTCGAGGACTTCCGAAAGAAGTATCAAAGAACCAACCAGGAGCTGGAGAAATTCAAAGACCTATTGTTG AAAGCAGAGACGGGGCGGAGCGCCCTGGACGTGAAGCTGAAGCATGCCCGTAATCAAGTGGACGTGGAGA TCAAGCGGAGGCAGCGCGCTGAGGCAGAGTGTGCAAAGCTGGAACAACAGATTCAGCTGATTCGAGACAT ACTCATGTGTGACACATCTGGCAGTATTCAGCTGAGCGAGGAACAAAAATCAGCTCTCGCTTTCCTCAAC CGAGGCCAAGCATCCAGTGGCCACGCCGGCAACAATAGACTGTCAACGATTGATGAATCTGGTTCCATTT TATCAGATATCAGCTTTGACAAGACTGATGAATCACTGGACTGGGATTCTTCTTTGGTGAAGAATTTCAA AATGAAGAAACGAGAGAAGAGGCGCTCCAACAGTAGACAGTTCATCGACGGCCCTCCTGGGCCTGTGAAG AAAACTTGTTCCATTGGCTCTACAGTAGACCAGGCAAATGAATCAATAGTTGCAAAAACTACAGTGACTG TTCCCAGTGATGGGGGACCCATTGAAGCTGTGTCTACTATTGAGACATTGCCGTCCTGGACCAGGAGTCG CGGGAAGTCAGGACCTTTACAACCTGTGAACAGTGACTCCGCTCTGAACAGCAGGCCACTGGAGCCAAGA ACTGACACAGACAATTTGGGCACACCTCAGAATACTGGAGGCATGCGCTTGCACGACTTCGTCTCAAAGA CGGTTATTAAGCCTGAATCTTGTGTTCCGTGTGGAAAGCGGATCAAGTTTGGCAAGCTGTCTCTGAAGTG TCGAGACTGTCGTTTGGTCTCCCATCCAGAATGTCGGGACCGATGTCCCCTTCCCTGCATCCCCCCCCTG GTGGGGACACCGGTTAAGATTGGAGAGGGCATGCTGGCCGACTTCGTGTCGCAGGCTTCTCCCATGATCC CTGCCATTGTCGTCAGCTGTGTCAATGAGATCGAGCAGCGAGGCCTGACTGAGGCAGGCTTGTACAGGAT CTCAGGCTGTGACCGCACAGTGAAAGAACTGAAAGAAAAATTCCTTAAGGTGAAAACTGTGCCCCTCCTC AGCAAAGTGGACGATATCCACGTCATCTGCAGCCTCCTGAAGGACTTCCTGCGCAACCTCAAAGAGCCCC TCCTGACCTTCTGGCTGAGCAAAGCCTTCATGGAGGCAGCAGAGATAACAGATGAAGACAACAGCACAGC CGCCATGTACCAGGCTGTCAGTGAGCTGCCCCAGGCCAACAGGGACACGCTAGCCTTCCTTATGATCCAC CTACAGAGAGTGTCTCAGAGTCCAGACACTAAGATGGATATTGCCAATCTAGCTAAAGTCTTTGGCCCTA CAATAGTTGCCCATACTGTGCCCAATCCAGATCCAGTGACAATGTTCCAGGACATCAAACGTCAGCTCAA GGTGGTGGAGCGCCTACTCTCTCTCCCCTTGGAGTACTGGAATCAGTTCATGATGGTGGACCAAGAGAAC ATAGACAGCCAGCGAGGCAATGGAAACTCAACACCACGCACACCAGACGTTAAAGTGAGCTTACTGGGGC CTGTGACCACTCCTGAATTCCAGCTTGTCAAGACTCCTTTATCAAGTTCCCTGTCACAGAGGTTGTACAA CCTCTCCAAGAGCACACCCAGATTTGGGAACAAGAGCAAGTCTGCCACCAACTTAGGTCAACAGGGCAAA TTTTTCCCTGCTCCGTACCTCAAGTAAAGCTGTGTCTGCCTGTGTTTACTGCACGAGACACCCCTGTCTG CTCTTCAGCCTCCTGTGTAATGACTACTTTTAGCATTTTCCAGACTTTAAATAAAGTTGAACGCGTATGA GAGTTTGAGTGTTGCATAGCTCCCTCCCACCTGGACACTTAGCACCTTTACTAGTTGTCGGGAGCTTTAA AATAGGAGATCTTTACCAGGGCCGAAGGGGAAATGGGTATAAGTTGGGGCTCTTAGGGGGAGGGGAGAGG AGATCCCTTTGGCTTAAAGCCAAATACTGCTCATGAAATGACTTTGCTGTGGTGTCACTTAGACAATGAC AGAAACTGTACCTCTTAGGTAACGTCTTAGTTATCTCAGGACAGGATAAAGAGCTGCGCAGCGGCCCCTT CTCAGAACACAGGCCTCTTCCTCCTTCGGGGACTGACAAGAAGCTTGGAGCCCTCAGCGGGATGGGCCGG GCCACTGATCTGAGGATGCTTCACTTCTGTCTGGGTTAAGCTGAGAAAGTGCCACAGGCTCTGATCTCAC ACAGCGTGCTGTTTCTAGCCAGCCTCTGCCTGGATTTTGCAGTCAAAGTCAGGTTGATCTGAAGCCGAAT TCGTTCTGATGCCTGACCCCCTTGTTTGTCCAGTGGAGCCCATTTAAAAAGTCAGCTAGCAGTTCAGAAG ACAATTTTCATATAGCAAGAAAGTTAGCTGCTTCTGTCTTTGGTGAAGTTTTTCTGTGAAACAGATCTAT ATATGTGAACATTGGCTTTTTTGGTTAGCATTTTCCAGTGTTCAAAATGGCTTCCTTCCCTGGGATGTTT TCTGACCCATACTAACCCTTACCTGTAACATGTATCTGGAATATTATGTGGAAAAAATAAATAGCTTTTT CAAAGTGAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC010715
• Insert Size: 1887 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010715, AAH10715
• RefSeq Size: 2913 bp
• RefSeq ORF: 1887 bp
• Locus ID: 26934
• Cytogenetics: 15 F1
• Gene Summary: Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.[UniProtKB/Swiss-Prot Function]