Glmn (NM_133248) Mouse Untagged Clone

CAT#: MC200446

Glmn (untagged) - Mouse glomulin, FKBP associated protein (Glmn), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Glmn
• Synonyms: 9330160J16Rik; AW227515; Fap48; Fap68
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003446 sequence for NM_133248
  GGAGGGCAAGAGGCAGGCGCGTGGCTCGGCGGTTCATTACAGCATCAAAATTAGGAAAATAATTAGGAAT GGCTGTGGAGGAACTTCAGAGCATAATAAAAAGATGTCAAATCCTAGAAGAGCATGATTTTAAAGAAGAA GATTTTGGCCTCTTTCAGTTAGCAGGTCAAAGATGCATTGAAGATGGTTATATAAACCAGCTGCTAGAGA TTATTCAAGATGAAAAGAACAAGACCATCATTAAGTCTATGGGGTGGAATCTTGTTGGTCCAGTTGTTCG ATGCCTCCTGAGGGGCAGAGAAGAGGATAAAAGAGAAGAGTGTTTTCTGATATTTGATTTGCTGGTGAAG TTATGTAATCCAAAGGAATTGTTGTTGGGTTTGCTTGAACTCATTGAAGAGCCCTCCGGAAAACAGATTT CCCAAATTATTCTTCTTTTACTGCAACCATTACAAACAGTTATTCAGAAACTTCCTAACAACAAGGCATA CTCCGTTGGACTAGCATTGTCAACACTTTGGAGTCAGCTGTCTCTTCTTCCTGTTCCACACTCAGAAGAA CAAATTCAGGCAGATGATTATGGCCTCTGTCAGTGTTGCAAGGCCTTGATAGAGTTCACGAAACCTTTTG TGGAAGAAGTAATAAGTGATAAAGAAAACAAAGAAAATGCAAAACTAAAAGATGAATTACTGAAATTTTG TTTCAAAGGCTTGAAATGCCCTTTGCTGACAGCACAATTCCTCGAACAGTCAGAAGACGTTGGAAATGAC CCTTTTCGGTGTTTTGCATCTGAAATAATAGGATTTTTATCAAAAATTGGACACCCTGTCCCCCAAATTA TTCTTAATCATGGAAGGAAAAAAAGGACTTGGGATTCCCTTGAATTTGAAGAAGAAGAAGACAAACAACT GGCAGAGTCTGTGGCTTCTCTGACATATCTAGTATTTGTTCAGGGCATCGGTATTGATCAGCTGCCCATG GTCTTAAGCCCATCGTACCTTCTGCAGTTGAACATGGAGCATATTGAAGTGTTTCTGCAAAGAACAGAAC AGTCTATTTACTCCAAAGGATTGGAACTTCTGGAGACTAGCTTATTGAGATTAGAAGACAACAGCCTATG TTATCAGTACTTGGAAATCAAGAGTTTTCTTGCTGTGCCTCAGGGCTTAGTCAAAGTTATGACACTTTGC CCCATTGAGACATTGAGGAAAAAAGGTTTATCTATGCTTCAGCTGTTTATTGACAAGTTGGATTCACAAG GCAAATATACATTATTTAGGTGCTTACTAAATACAAGTAATCACTCAGGAGTGGAAGCCTTTGTAATTCA AAACATCAAAAATCAGATTGATTTATCATTTAAGAAAACATATAACAAATGGTTTGCAGGAGCACAGCTG ATCTCTCTGTTAGACCTGGTCCTGTCTCTCCCTGAGGGCGCTGAGACAGACTTACTGCAGAACTCAGACA GGATTATGGCTTCATTAAATTTATTGAGGTATTTGGTTATCAAAGATAATGAAGATGACAATCAAACTGG ATTATGGACAGAACTTGGAAAAATTGAAAATAACTTTCTAAAGCCACTTCATATAGGACTTAATATGTCA AAAGCACATTATGAAGCAGAAATTAAAAACAGCCAACAAAATAACCAAGTAGCCTCGATGTGTAAAGGTG TTTGTTCCGTGACTGTAGGTGGAGAAGAAATCCCTTCTATGCCTCCTGAAATGCAGCTTAAGGTCTTACA TTCCGCTCTCTTCACATTTGACTTGATTGAAAGTGTTCTGGCTCGAGTAGAAGAACTCATTGAAATCAAA TCAAAGTCTACCTCTGAAGAAAATGTTGGGATAAAGTAAAGGTTCTATTTCATAAATAAAAACCAACAAA ATGCAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_133248
• Insert Size: 1791 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003446, AAH03446
• RefSeq Size: 1911 bp
• RefSeq ORF: 1791 bp
• Locus ID: 170823
• UniProt ID: Q8BZM1
• Cytogenetics: 5 E5
• Gene Summary: Regulatory component of cullin-RING-based SCF (SKP1-Cullin-F-box protein) E3 ubiquitin-protein ligase complexes. Inhibits E3 ubiquitin ligase activity by binding to the RING domain of RBX1 and inhibiting its interaction with the E2 ubiquitin-conjugating enzyme CDC34. Inhibits RBX1-mediated neddylation of CUL1 (By similarity). Required for normal stability and normal cellular levels of key components of SCF ubiquitin ligase complexes, including FBXW7, RBX1, CUL1, CUL2, CUL3, CUL4A, and thereby contributes to the regulation of CCNE1 and MYC levels (PubMed:22405651). Essential for normal development of the vasculature (PubMed:22405651). Contributes to the regulation of RPS6KB1 phosphorylation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Both variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.