Raly (NM_023130) Mouse Untagged Clone

CAT#: MC200444

Raly (untagged) - Mouse hnRNP-associated with lethal yellow (Raly), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Raly
• Synonyms: AI663842; Merc
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC004851 sequence for NM_023130
  GCGCGCGAGCGGCGCGGGGCGCGGAGCCGAGGGAAGCCGAGGGGGCGGAAGCGGTCGCGACTCTCGCGCG TGTGCTCGGGCTCCTCACGCGGCGGCCAGGGCCGCCTCTTCCCTCCCGCCCTCCGAGAGCAGACGCGCCG TCGCCCTTCGGTGCCGCGCGGCTTCCTCCAGACCTCGGCGCGGGTGAACACCATGTCCTTGAAGATTCAG ACCAGCAATGTAACCAACAAGAATGACCCTAAGTCCATCAACTCTCGGGTCTTCATCGGAAATCTAAACA CAGCTGTGGTGAAGAAGTCAGATGTGGAGACCATCTTTTCCAAGTACGGCCGAGTGGCTGGTTGCTCTGT GCACAAAGGCTATGCTTTTGTCCAGTATGCCAATGAGCGCCATGCCCGGGCAGCTGTGCTGGGAGAGAAT GGGCGGGTGCTGGCTGGACAGACCCTGGACATCAACATGGCTGGAGAGCCCAAGCCTAATAGACCCAAGG GGCTAAAGAGAGCAGCAACTGCCATCTACAGGCTGTTTGATTATCGAGGCCGCCTTTCTCCAGTGCCTGT GCCCAGGGCAGTTCCAGTGAAGCGACCCCGTGTTACAGTCCCTTTGGTTCGCCGTGTCAAAACTACGATA CCTGTCAAGCTCTTTGCCCGCTCCACAGCTGTCACTACTGGCTCAGCCAAAATCAAGTTAAAGAGCAGTG AGCTACAGACCATCAAAACAGAGCTGACACAGATCAAGTCCAACATCGATGCCCTGTTGGGTCGCTTGGA ACAGATTGCTGAGGAACAGAAGGCCAACCCAGATGGCAAGAAGAAGGGTGACAGCAGCAGTGGTGGAGGA GGAGGCAGCAGTGGTGGAGGCGGCAGTAGCAATGTTGGTGGTGGCAGCGGCGGCGGCAGCGGGAGCTGCA GCAGCAGCAGCCGGCTACCAGCGCCCCAAGAAGACACGGCTTCTGAGGCAGGCACACCCCAAGGAGAAGT CCAAACTCGAGATGATGGTGATGAGGAGGGACTGCTAACACATAGCGAGGAGGAGCTGGAGCACAGCCAG GACACAGATGCAGAAGATGGAGCCTTGCAGTAAGCAGCTTAACAGGAGCATTGGCCACCAGCAGAAGGGC ATCACTGTCTCAGGCCTCAAGCCAGGCACCCATCTCTGGATGCCAGTCTATAGCGGGTACCAGAGGAAAG CTGGCAGCAGTAACTCTCTCCCCATGCATCCTAGCCAGTGAGTGCTACATCCTTTGCAAGTGGAGTTACT GGCCTACCCTTACCCCATGCATTCTTCCTGTCTGCACTGCCTGGGCCAAGGGGCAGAAACACTCTGCTCT TCTTCCCCAGGACATTCCCAGGCTTGGGGTTTTTCTATAGGTTTGAAAGTAAAGGGGGGAGGGTGGGAAG GGTGGGAGGAACCTGACAATAAAGAGATTGGATCCAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_023130
• Insert Size: 891 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC004851, AAH04851
• RefSeq Size: 1450 bp
• RefSeq ORF: 891 bp
• Locus ID: 19383
• UniProt ID: Q64012
• Cytogenetics: 2 76.83 cM
• Gene Summary: RNA-binding protein that acts as a transcriptional cofactor for cholesterol biosynthetic genes in the liver (PubMed:27251289). Binds the lipid-responsive non-coding RNA LeXis and is required for LeXis-mediated effect on cholesterogenesis (PubMed:27251289). May be a heterogeneous nuclear ribonucleoprotein (hnRNP) (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has an additional exon in the 5' UTR and lacks an exon in the 5' coding region, compared to variant 1. It encodes a protein that is 16 amino acids shorter than the long isoform.