Pcca (NM_144844) Mouse Untagged Clone

CAT#: MC200415

Pcca (untagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), (10ug)


  "NM_144844" in other vectors (4)

Reconstitution Protocol

USD 664.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

USD 507.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Other products for "Pcca"

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Pcca
Synonyms C79630
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC006915 sequence for NM_144844
CGGACGCGTGGGGCCGGCAAGTCTGCGGGGTGGACGATGGCGGGGCAGTGGGTCAGGACCGTGGCGCTGT TGGCGGCCAGGCGGCATTGGCGGCGGTCCTCGCAGCAGCAATTGCTGGGGACGCTGAAGCATGCTCCAGT CTATTCATACCAATGCCTAGTGGTGTCCAGAAGTCTCAGTTCTGTGGAATATGAGCCTAAAGAAAAGACT TTTGATAAAATTCTCATTGCTAACAGAGGAGAAATTGCCTGTAGGGTTATTAAAACTTGCAAGAAGATGG GCATCAAGACAGTTGCCATTCACAGTGATGTTGATGCCAGTTCTGTTCACGTGAAAATGGCGGATGAGGC TGTCTGTGTTGGCCCAGCTCCCACCAGTAAAAGCTACCTCAACATGGATGCCATCATGGAAGCCATTAAG AAAACCAGGGCCCAAGCTGTACACCCAGGGTATGGATTCCTGTCAGAAAACAAAGAGTTTGCAAAGCGTC TGGCAGCAGAAGATGTCACTTTCATTGGACCTGATACTCATGCTATTCAAGCCATGGGTGACAAGATAGA AAGCAAACTATTAGCCAAGAGAGCAAAGGTCAACACAATCCCTGGTTTTGATGGGGTAGTAAAGGATGCA GATGAAGCTGTCAGAATTGCAAGGGAAATTGGCTACCCTGTGATGATCAAGGCCTCAGCAGGCGGTGGTG GGAAAGGCATGCGCATCGCCTGGGATGACGAAGAGACCAGGGATGGCTTTAGATTTTCATCCCAGGAAGC TGCTTCTAGTTTTGGTGATGATAGACTACTAATAGAAAAATTTATCGATAACCCTCGTCATATAGAAATC CAGGTTTTAGGGGATAAACATGGCAATGCTCTGTGGCTCAATGAGAGGGAGTGCTCGATCCAGAGAAGGA ACCAGAAGGTGGTAGAGGAGGCGCCAAGCATTTTTCTGGATCCTGAAACTCGCCAAGCAATGGGAGAGCA GGCCGTGGCTTTGGCTAAAGCCGTGAAGTATTCTTCTGCTGGAACTGTGGAATTTCTTGTGGACTCCCAG AAGAATTTTTACTTCTTGGAGATGAATACAAGACTACAGGTCGAACATCCTGTCACAGAGTGCATTACTG GCCTGGACTTAGTCCAAGAAATGATCCTTGTTGCTAAGGGTTACCCACTCAGGCACAAGCAAGAGGATAT TCCCATCAGTGGCTGGGCAGTTGAATGTCGGGTTTATGCTGAGGACCCCTACAAGTCTTTCGGTTTACCG TCTATTGGGAGGCTGTCCCAGTACCAAGAGCCGATACATCTACCTGGTGTCCGAGTTGACAGTGGCATCC AACCAGGAAGTGACATCAGCATCTATTATGATCCTATGATTTCAAAGCTAGTCACATATGGGTCTGACAG AGCAGAAGCCCTGAAGAGGATGGAAGACGCACTGGACAATTATGTGATCCGGGGTGTTACACACAACATC CCATTGCTCCGGGAGGTGATAATCAACACACGTTTTGTGAAAGGAGACATCAGCACTAAGTTTCTCTCTG ATGTGTATCCTGATGGCTTCAAAGGGCACACGTTAACACTGAGTGAGAGAAACCAGTTATTGGCCATTGC ATCATCTGTATTTGTGGCATCCCAGCTACGAGCTCAGCGCTTCCAAGAACGTTCAAGAGTACCAGTTATT AGGCCTGATGTGGCTAAGTGGGAGCTCTCGGTAAAGTTACATGATGAAGATCATACTGTCGTGGCATCTA ACAATGGGCCGGCATTTACCGTGGAAGTTGATGGCTCGAAACTAAATGTGACCAGTACGTGGAACCTGGC GTCACCCTTATTGTCTGTCAACGTTGATGGCACGCAGAGGACTGTGCAGTGTCTTTCTCGGGAAGCAGGT GGAAACATGAGCATCCAGTTTCTTGGCACAGTGTACAAAGTGCACATTTTAACCAAGCTTGCTGCAGAGC TGAACAAATTCATGCTTGAAAAAGTGCCCAAGGACACCAGCAGCACTCTGTGCTCCCCGATGCCTGGAGT GGTGGTGGCCGTTTCTGTCAAGCCTGGAGACATGGTAGCAGAAGGTCAGGAAATCTGTGTGATTGAAGCT ATGAAAATGCAGAACAGTATGACAGCTGGGAAAATGGGCAAGGTGAAATTGGTGCACTGCAAAGCTGGAG ACACAGTTGGTGAAGGAGACCTGCTTGTGGAGCTGGAATGAAGTATTTCTAGCCTTTCCGTCAGCACTGC TCACTGCTTTAACTGGCGTCTCCATTATTCTCTAACCCTCAATTTGCGCAAGCATTTACAGGAGCACTCC TGTGCAGGAGGTTCTACAGCCTCCTGTTTATTCCTCAGAAGAGGCAAAACCGACATTCTGCCAACAAATC ACCAATGGAAATTCTTGATATAAATACTTGTACATATGATTTGTACTTCTGCTGAGATTTCCTAGTGTCA AAATTAAATCAATAAAACCAAGCATTTGTCTAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_144844
Insert Size 2175 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC006915, AAH06915
RefSeq Size 2499 bp
RefSeq ORF 2175 bp
Locus ID 110821
UniProt ID Q91ZA3
Cytogenetics 14 65.99 cM
Gene Summary This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (By similarity). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA (By similarity). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).[UniProtKB/Swiss-Prot Function]

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.