2010301N04Rik (BC002179) Mouse Untagged Clone

CAT#: MC200315

2010301N04Rik (untagged) - Mouse RIKEN cDNA 2010301N04 gene (cDNA clone MGC:7351 IMAGE:3487226), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 2010301N04Rik
• Synonyms: 2010301N04Rik; AI452191
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC002179
  CGGACGCGTGGGCGGACGCGTGGGCTCACCAGGAAAGGAGCCTCGGGTTTCACACCCCTTCGGGCCATGC CCAGCGTGCCTGAGGGAGGTGGCTACGAGATAACAGACAAAAGAGTATCCAGAAGACATGCCATTCTTGA AGTGGTTGATAGTCAGCTTCGAATCAAGCCAAATCTGAAGTGGAGATGGAGTGTACTCTGAGAAACAGTC AGATGCTTGATGAAGATGATATATTGAGTGAGATGCAGAAGTCCCCTGTGGTTAACTTACCTGATAAGAC AACTGGTGCCTCACAGCTGCAAGGAAGCCCTGAAATAACCAAGACCAAGTGTCCCACCATAGATCCTATG TCCTCTTCAGGTGAATGCAGAGCTTTCAGTGAGCACCAGCCACATCCTACCCAGAGGAAAAGAATCCTCC CAGCTTGGATGTTGGCGGAAAGTCTAAGTGACCAGAGCCTCTCCACACCAGCTGAGGGGGGAGATAAGGA TGTCATCCAGAGGAGTGGAAAAGCAGGAACCTGTGAAGATAGAACCCCAGGAAACACATCATGGCATGGG AAAAAGCGGCTTAGTCCATCAGGAAATTCAAAAAGTGTGTCAGCGGAACAAGACCCTGGGAAGAAGTGCA GAAAGGCTGACCAAGAAGGACCGGGTGTTTCTTCTGAGAATGTTCCAGAATCATCATCTTCAAACATAGT AAAGGACCCAGATGTGGATATTGTGAAGACAAATAAACAGAAGGACGGGATTTTAATAGAGGAACTTGGT GAGGTCTCTAAACATAAAGCCGCCACCAAACCAACCACAAATGAAGAGGGCGAGAGTTGTGCACGTGTCC AAAGCAAGTCACCTCCTGAGAAATCTCAAGGATGCCATCCTGAGTCTAGCTCTGCTCCCTCCAGTCCTGA TGCTTTGCACACAGACACAGCTGACCCAGTGCTGGGCTGTTCAGAAGAAAGCAAGGTCCGGAGGACGGCC TGCATGTATGGGGCCAACTGCTACAGGAGGAACCCCCTCCACTTTCAACACTTCAGCCACCCTGGTGACA GTGACTATGGAGAGGTACATGGCACAGATGAAGGTGTGATTGGTGACAGGCCCGAGTGTCCCTATGGAGC GTCTTGTTACAGGAAGAACCCCCAGCACAAGATGGAATATAGGCACAGTGCACTTCCAGCCAGAGTTGCT CTGGATGAAGATGACGATGATGTCGGGCAACCCAGTGACGATGAGGACGAGGAAGACTATGAGCCGACAG ATGAAGATTCTGATTGGCACCCAGGAAAGGACGATGAGGAGCAGGAAGATGTGGATGAGCTTTTGAAAGA AGCAAAAAGGTTTATGAGAAGGAAAAAGTAGCGTTTCTGCAATGACTCACATTTGCTTTTTTTCTCTGTG GGAAACATACTCATTTTTTTTTTCTTTTAATAGTTCTTTGCACTTGAAACATTGAAACACCAGCCTTCAG TGAAATAGGTGGATGTATGCTATTGCCTATTTTGGTGCCTATTTAATGTATCCATCAGTGAAAGCAGGTA AAGAGGTTGGGCAGTCTGATATTTTCTAAATACTTTACATACTGCGAATAAAAAGCCATAGGTTGTACAA AAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC002179
• Insert Size: 1176 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC002179, AAH02179
• RefSeq Size: 1623 bp
• RefSeq ORF: 1176 bp
• Locus ID: 72103
• Cytogenetics: 6 D1
• Gene Summary: Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ).[UniProtKB/Swiss-Prot Function]