Klhl22 (BC005800) Mouse Untagged Clone

CAT#: MC200279

Klhl22 (untagged) - Mouse kelch-like 22 (Drosophila) (cDNA clone MGC:6948 IMAGE:2812589), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Klhl22
• Synonyms: 2610318I18Rik; Kelchl
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC005800
  GGCGCACGCCGGTAGGATCTGCCTCCCAAGTGCTGGGATTAAAGGCATGAGCCACCACTGCCCGCTGACT ACAGTAGTGAGGGCTGCTGTGGCTGTCAGGATGGCTGAGGAGCAGGACTTTGCCCAGCTGTGCAGGCTGC CCACACAGCCCTCCCACTCCCACTGTGTCAACAATACCTATCGAAGCACACAGCACTCCCAGGCCCTGCT CCGGGGCCTGCTGGCTTTGCGAGACAGTGGGATCCTCTTTGACGTTGTCCTGGTGGTAGAGGGGAAGCAC ATTGAGGCCCATCGGATTCTGCTAGCTGCATCGTGTGATTATTTCAGAGGAATGTTTGCTGGGGGATTGA AGGAGATGGAGCAGGAAGAGGTCTTGATCCACGGTGTGTCTTACAATGCCATGTGCCAAATCCTGCACTT CATATACACTTCTGAACTAGAGCTCAGCCTGAGCAATGTGCAGGAGACCCTGGTTGCTGCCTGCCAACTT CAGATACCAGAAATTATCCACTTCTGCTGTGATTTCCTCATGTCATGGGTAGATGAGGAGAATATCCTTG ATGTCTACCGGCTAGCAGACCTCTTTGACTTGAACCATCTGACCCAACAGCTAGATACCTACATCCTCAA AAACTTTGTGGCCTTCTCCAGAACTGACAAGTACCGCCAGCTTCCCTTGGAGAAGGTCTACTCTCTCCTC AGCAGCAATCGCCTGGAGGTTTCCTGTGAGACTGAAGTGTATGAAGGGGCCCTGCTCTACCATTACTCCC TAGAGCAGGTGCAGGCTGACCAGATCTCACTGAATGAGCCCCCCAAGCTCCTAGAGACTGTGCGCTTTCC CCTGATGGAAGCTGAGGTCCTTCAGCGGCTGCATGACAAGCTTGGTCCTAGCCCACTGAGGGACACAGTG GCCAGTGCCCTTATGTACCACCGGAATGAGATCCTGCAGCCCAGCCTTCAGGGCCCACAGACAGAGCTTC GCTCGGACTTCCAGTGTGTTGTGGGCTTTGGGGGTATTCACTCCACACCGTCTACAATCCTCAGTGACCA GGCCAAGTACCTGAATCCTCTACTGGGAGAGTGGAAACACTTCACTGCCTCTCTGGCGCCCCGAATGTCC AATCAGGGCATTGCAGTGCTCAACAACTTCGTGTACTTAATTGGAGGGGACAATAATGTCCAAGGATTTC GAGCTGAGTCCCGATGCTGGAGGTATGACCCAAGGCACAATCGCTGGTTCCAGATCCAGTCCTTGCAGCA AGAACATGCAGACCTATGTGTGTGTGTAGTGGGCAAGTACATCTATGCTGTGGCAGGCCGTGACTACCAC AATGACCTGAGTGCTGTGGAGCGCTATGACCCTGCCACCAACTCCTGGGACTATGTGGCCCCACTCAAGA AGGAGGTATATGCCCATGCAGGCACCACACTGCAGGGGAAGATGTATATTACCTGTGGTCGCAGAGGAGA GGACTACCTGAAAGAGACACACTGCTATGACCCAGGAAGCAACACGTGGCATACTCTGGCTGACGGGCCT GTGCGACGGGCCTGGCATGGCATGGCTGCCCTCCTCGACAAGCTGTTTGTGATTGGAGGCAGCAACAATG ATGCTGGCTACAGAAGGGATGTGCACCAGGTGGCTTGCTACAGTTGTACATCTCGTCAGTGGTCATCGGT CTGCCCACTCCCAGCTGGGCATGGTGAGCCTGGCATTGCTGTGCTAGACAGCAGGATATATGTGCTGGGT GGCCGCTCTCACAACCGAGGCAGCCGTACAGGCTACGTGCACATCTACGACATGGAGAAAGACTGCTGGG AAGAGGGACCCCAGCTGAACAATTCCATCTCAGGCCTGGCAGCCTGTGTGCTCACCCTGCCTCGATCCCT GCTCCATGAGCAACCCCGAGGGACCCCCAACCGTAGCCAGGCAGATGCAGACTTTGCTTCTGAGGTGATG AGTGTGTCTGACTGGGAAGAGTTTGACAACTCTAGCGAGGACTAGGGCTCTCCCCAGTGTTTGGGTGTTC AGGAAGGGTAATGGGCAGTGAAGGCCGTAGAGCCTGGGTTAGAACACCAGGCTCTAGGGGAGGGGCCCCT CCTTAAGCCATCCTGGTTCACCAGCTGCCCCTTTCACTCTGTTTCTTCAACCAGCCAGCTGCCAGCATTT GTAAACTCTGTGGCTGTGAATTACATTACAAAGAAAAGTGCTGGGGGGAAGGCTGCTATCATCAGAGGGA ATGACCCATCCTCTATAAACTCTGAATGAGTGGGATCTATTTGGCCGCTTCTTCTCCCTAGTCTGGTCCC TGATGGGTTAAGTAAGGTCCTTCTTTCAAGAGATCCTGGGGAGAGCACTCCTAGCCATAGCCAAAGTTTG TGGCTCCCTTTCTGTGAACCGTGGTCAGTCTGGTGAGCAGCGCCAGCTGCCTGCAGCCAGGGCCTTGAGC ACTGGGGAGCTCTCAAGGTATACTCTTAGAGTAACTATATACCTGTAACTGGACCTGGAGATGAAGAGAT ACCCTCATTGCTCTGTGTCTGACCCAATATTGTTGAAAAATGAAATAAAGCATTATGCAAAAGGTGTTAC ATCTAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC005800
• Insert Size: 1905 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005800, AAH05800
• RefSeq Size: 2610 bp
• RefSeq ORF: 1905 bp
• Locus ID: 224023
• Cytogenetics: 16 11.01 cM
• Gene Summary: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation (By similarity). The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway. It is therefore an amino acid-dependent activator within the amino acid-sensing branch of the TORC1 pathway, indirectly regulating different cellular processes including cell growth and autophagy (PubMed:29769719).[UniProtKB/Swiss-Prot Function]