Pdlim1 (NM_016861) Mouse Untagged Clone

CAT#: MC200210

Pdlim1 (untagged) - Mouse PDZ and LIM domain 1 (elfin) (Pdlim1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pdlim1
• Synonyms: Clim1; CLP36; mClim1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC004809 sequence for NM_016861
  CCGGGATCCCCGCGCGCCGTGCAGTTCTGTGCCTTGCCTGTCTGTCCCTTCCGATTGTGCCTAGCGATGA CCACCCAGCAGATTGTTCTCCAGGGCCCGGGTCCCTGGGGTTTCCGCCTCGTGGGCGGCAAGGACTTCGA ACAACCTCTCGCCATTTCCCGGGTCACTCCCGGAAGCAAGGCTGCTATAGCGAATTTATGTATTGGAGAT TTAATCACAGCCATCGATGGGGAAGATACCAGCAGCATGACACACTTGGAAGCTCAGAACAAAATCAAAG GCTGCGCAGACAACATGACGCTCACAGTATCCAGGTCTGAACAGAAGATTTGGTCTCCTCTAGTGACCGA GGAGGGGAAACGTCATCCCTACAAGATGAATTTAGCGTCGGAACCCCAAGAAGTCCTGCACATAGGAAGT GCCCACAACCGAAGTGCCATGCCATTTACTGCCTCACCTGCTCCCAGCACCCGGGTCATCACAAACCAGT ACAACAGCCCAACTGGCCTCTACTCATCCGAAAACATCTCTAACTTCAACAATGCTGTGGAGTCGAAGAC CTCAGCCAGTGGGGAGGAGGCGAACAGCAGGCCCGTGGTTCAGCCCCATCCCTCCGGCAGCCTTATCATT GACAAGGACTCTGAAGTTTACAAGATGCTTCAGGAGAAGCAAGAATTAAACGAGCCCCCCAAACAGTCCA CATCCTTCCTGGTTCTGCAGGAGATCCTGGAGTCAGATGGGAAAGGGGATCCCAACAAGCCATCAGGATT TAGAAGTGTTAAAGCTCCAGTTACCAAAGTGGCTGCATCTGTTGGAAATGCTCAGAAGCTGCCCATCTGT GACAAATGTGGAACTGGTATTGTTGGTGTGTTTGTGAAGCTGAGGGATCACCATCGCCACCCTGAATGCT ATGTGTGCACCGACTGTGGCATCAACCTGAAACAGAAGGGCCATTTCTTTGTAGAGGATCAGATCTACTG TGAAAAGCATGCCCGGGAGCGGGTCACTCCGCCTGAGGGCTATGATGTGGTCACAGTGTTCCGCGAGTGA TGGAGAGGACCTGCCCTGTCTACTGTCCTCCAGCCAGCCTGCTGCACCTGGTTCTCGGAATATTCTGGCT CTCTCCTCCCTGACAGTCCTGTGCTTACTTAGTGTTAGTCATGCGCAATAAACATTGGGTTTTGTCTGGG CTGACTGACTCCCACTGGCAGTGTGACCTCTGCTTTTACATTATTGGGAGATGGTTTTGTCCCTGTCCCT TCACCCACACCTCAGTGCACCTTCTTCACTTGGAGTCTTCTCTGCTGCAAATGGACATTGGTCCATTGGA ACTAAGCCAAATGTAGTATCTCTGATGTTACTTGGTTTTACTTAATAAATTTAGACTTTAAAGCAAAAAA AAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_016861
• Insert Size: 984 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC004809, AAH04809
• RefSeq Size: 1413 bp
• RefSeq ORF: 984 bp
• Locus ID: 54132
• UniProt ID: O70400
• Cytogenetics: 19 C3
• Gene Summary: Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton (By similarity). Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts (By similarity).[UniProtKB/Swiss-Prot Function]