Nus1 (NM_030250) Mouse Untagged Clone

CAT#: MC200036

Nus1 (untagged) - Mouse nuclear undecaprenyl pyrophosphate synthase 1 homolog (S. cerevisiae) (Nus1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nus1
• Synonyms: 1600027K07Rik; AU019165; AW538011; BC003223; D10Ertd438e
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003223 sequence for NM_030250
  CCCACGCGTCCGGTTGGCAGTGGCGAGGGGCTCGGGCGCGGGGGGTGGGGGGACGCGGAGCGATGGCCCG CGCCGGCCGCTGGGGCGGATAAGACCCTGTCGCGCCGCGGGTGTGGGCCGGAGGGAGGTGACGGTGCCCG AGCGCCACAGGAGTATGACGGGGCTGTACGAGCTGGTGTGGCGGGTGCTGCACGCGCTGCTCTGCCTGCA CCTCACGCTCACCTCCTGGCTCCGCGTTCGCTTCGGCACCTGGAACTGGATCTGGCGGCGCTGCTGTCGC GCCGCCTCCGCCGCGGTCCTAGCGCCGCTCGGCTTCACGCTCCGCAAGCCCCGGGCCGTCGGCAGGAACC GGCGTCATCACCGGCACCCGCACGGGGGACCGGGACCGGGACCGGGACCGGCCGCCACCCATCCTCGGCT GCGCTGGCGCGCGGACGTCCGGTCCCTGCAGAAGCTGCCGGTGCACATGGGCCTGTTGGTCACGGAGGAA GTGCAGGAGCCCAGCTTCTCAGACATCGCCAGCCTCGTGGTGTGGTGTATGGCCGTGGGGATCTCCTACA TTAGCGTCTACGACCACCAAGGTATTTTCAAGAGAAATAATTCCAGATTGATGGATGAAATTTTAAAACA GCAACAGGAACTTTTGGGCCAAGATTGTTCCAAATACTCAGCAGAGTTTGCAAATAGTAATGACAAAGAC GATCAAGATTTAAATTGCCCTTCGGCAGTGAAGGTGCTGTCCCCAGAAGATGGAAAAGCGGATATTGTGA GAGCTGCCCAGGATTTTTGCCAGTTAGTAGCCCAGCAGCAGAGGAAGCCCACAGATCTGGATGTAGATCT GCTAGGCAGCTTACTTAGTTCACATGGGTTCCCTGATCCTGACTTAGTGTTGAAGTTTGGTCCTGTGGAC AGCACATTAGGCTTTCTTCCCTGGCAAATCAGATTGACTGAGATCGTCTCTCTGCCTTCTCACCTAAACA TCAGCTATGAGGACTTCTTCTCCGCCCTTCGGCAGTATGCAGCTTGCGAACAGCGACTGGGAAAGTAATG GTCCCTACTTGCATAATTTGACTTCAGGCTTTTGGAGAAAAAGGACCCAAGTGACACTGATGTTTACAAA GTACCTATAAAACCCTGTACACACCTAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_030250
• Insert Size: 894 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003223, AAH03223
• RefSeq Size: 1168 bp
• RefSeq ORF: 894 bp
• Locus ID: 52014
• UniProt ID: Q99LJ8
• Cytogenetics: 10 26.64 cM
• Gene Summary: With DHDDS, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Both subunits contribute to enzymatic activity, i.e. condensation of multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol phosphate which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER). Regulates the glycosylation and stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol. Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator.[UniProtKB/Swiss-Prot Function]