Human Ankyrin repeat domain containing protein 65 (ANKRD65) (NM_001145210) AAV Particle

CAT#: RC227500A1V

AAV ORF Particles, serotype AAV-2, ANKRD65 (Myc-DDK-tagged)-Human hypothetical protein LOC441869 (LOC441869), transcript variant 1. Note: ORF is codon optimized, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Ankyrin repeat domain containing protein 65
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227500 ORF sequence, codon optimized.
  Due to the complexity of NM_001145210, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCCCAGAGGCCAGAACCTAGGGAAGAGGAGGAAGAGGAGCAAGAATTGCGGTGGATGGAGCTCG
  ATAGTGAGGAGGCCTTGGGTACACGAACCGAAGGACCCTCCGTGGTCCAGGGTTGGGGCCATTTGTTGCA
  GGCAGTGTGGAGAGGACCCGCCGGGCTGGTCACTCAACTCCTGAGGCAGGGAGCCTCTGTGGAGGAGAGA
  GATCACGCCGGACGCACTCCTTTGCACCTCGCTGTGCTTCGAGGGCACGCCCCCTTGGTCAGATTGCTCC
  TGCAGCGGGGTGCCCCAGTGGGAGCCGTAGATAGAGCTGGCAGGACCGCCCTGCACGAGGCCGCATGGCA
  CGGTCACTCCCGGGTGGCCGAACTCCTTCTTCAGAGAGGTGCCTCTGCCGCTGCCCGATCCGGCACCGGA
  CTGACACCATTGCATTGGGCCGCTGCCCTCGGGCACACACTTCTGGCTGCTAGACTCCTGGAGGCCCCCG
  GTCCCGGCCCTGCAGCGGCGGAAGCTGAAGACGCGAGAGGTTGGACCGCCGCGCATTGGGCAGCCGCCGG
  AGGGCGCTTGGCTGTCCTGGAGTTGCTGGCCGCCGGAGGCGCTGGCCTGGACGGGGCCCTGCTGGTTGCT
  GCAGCAGCCGGGAGGGGAGCTGCCCTTAGATTTCTGCTTGCTCGAGGAGCCCGGGTGGATGCGAGAGACG
  GGGCCGGAGCCACCGCACTGGGTTTGGCAGCCGCTCTCGGCAGAAGTCAAGATATAGAGGTCCTGCTGGG
  ACATGGCGCAGATCCTGGGATTAGAGATAGGCATGGAAGATCTGCGCTGCATAGGGCCGCAGCTAGAGGA
  CACCTTCTGGCAGTGCAGCTGTTGGTTACACAAGGGGCCGAGGTGGACGCCAGGGATACTCTGGGGCTGA
  CGCCCTTGCACCATGCTAGCAGAGAGGGACATGTGGAGGTGGCTGGATGCCTGCTTGATAGAGGCGCTCA
  GGTGGATGCCACAGGGTGGCTTAGAAAAACCCCTCTGCACCTGGCTGCCGAGAGAGGACATGGTCCCACT
  GTCGGACTGCTGCTCAGCAGGGGGGCAAGCCCGACTCTGAGGACCCAGTGGGCCGAAGTGGCGCAGATGC
  CAGAGGGCGACCTTCCACAGGCCCTCCCAGAGCTTGGGGGGGGCGAGAAAGAGTGTGAAGGAATCGAGTC
  AACTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227500 representing NM_001145210
  Red=Cloning site Green=Tags(s)
  
  MDSQRPEPREEEEEEQELRWMELDSEEALGTRTEGPSVVQGWGHLLQAVWRGPAGLVTQLLRQGASVEER
  DHAGRTPLHLAVLRGHAPLVRLLLQRGAPVGAVDRAGRTALHEAAWHGHSRVAELLLQRGASAAARSGTG
  LTPLHWAAALGHTLLAARLLEAPGPGPAAAEAEDARGWTAAHWAAAGGRLAVLELLAAGGAGLDGALLVA
  AAAGRGAALRFLLARGARVDARDGAGATALGLAAALGRSQDIEVLLGHGADPGIRDRHGRSALHRAAARG
  HLLAVQLLVTQGAEVDARDTLGLTPLHHASREGHVEVAGCLLDRGAQVDATGWLRKTPLHLAAERGHGPT
  VGLLLSRGASPTLRTQWAEVAQMPEGDLPQALPELGGGEKECEGIESTG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_001145210
• ORF Size: 1197 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_001145210.1, NP_001138682.1
• RefSeq Size: 2205 bp
• RefSeq ORF: 1200 bp
• Locus ID: 441869
• UniProt ID: E5RJM6
• Cytogenetics: 1p36.33
• MW: 41.5 kDa