Human RPP40 (NM_006638) AAV Particle

CAT#: RC219319A1V

AAV ORF Particles, serotype AAV-2, RPP40 (Myc-DDK-tagged)-Human ribonuclease P/MRP 40kDa subunit (RPP40), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: RPP40
• Synonyms: bA428J1.3; RNASEP1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219319 representing NM_006638
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACGCTGCGCCGGCTTCGGGAGGCGCCGCGGCACTTACTGGTTTGCGAGAAATCCAACTTCGGCA
  ACCACAAGTCGCGCCACCGGCATCTTGTGCAGACGCACTACTATAACTACAGGGTTTCATTTCTCATTCC
  TGAATGTGGGATACTATCGGAAGAACTGAAAAACCTGGTCATGAACACTGGACCCTATTACTTTGTGAAG
  AATTTACCTCTTCATGAATTAATTACACCTGAATTCATCAGTACCTTTATAAAGAAAGGTTCTTGCTATG
  CACTAACATACAATACACATATTGATGAAGATAATACTGTTGCCCTGCTACCAAATGGGAAATTAATTTT
  GTCACTGGATAAAGACACTTATGAAGAAACTGGACTTCAGGGTCATCCATCTCAGTTTTCTGGCAGAAAA
  ATTATGAAATTTATTGTTTCCATTGATTTGATGGAATTATCCTTAAACTTGGATTCTAAGAAGTATGAAA
  GAATATCTTGGTCTTTCAAAGAAAAGAAGCCATTGAAATTTGATTTTCTTTTGGCTTGGCATAAAACAGG
  TTCAGAAGAATCGACAATGATGTCATATTTTTCCAAGTACCAAATTCAGGAGCATCAGCCAAAAGTAGCA
  CTGAGCACGTTGAGAGATCTCCAGTGCCCAGTGCTGCAGAGCAGCGAGCTGGAGGGAACGCCAGAGGTGT
  CCTGCCGGGCTCTGGAGCTCTTCGACTGGCTCGGCGCCGTCTTCAGTAATGTCGACCTAAATAATGAGCC
  TAATAATTTCATATCAACCTATTGCTGTCCTGAGCCAAGCACAGTGGTGGCAAAAGCTTATTTGTGTACA
  ATCACTGGCTTCATACTTCCAGAGAAGATCTGTCTCCTATTGGAACATCTCTGTCACTACTTTGATGAAC
  CGAAGTTAGCTCCATGGGTTACACTGTCCGTTCAAGGCTTTGCAGACAGCCCTGTTTCTTGGGAAAAAAA
  TGAACATGGTTTTCGAAAAGGAGGAGAACATTTATATAACTTTGTGATTTTTAATAATCAGGACTATTGG
  CTTCAGATGGCTGTTGGGGCAAATGATCACTGTCCACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219319 representing NM_006638
  Red=Cloning site Green=Tags(s)
  
  MATLRRLREAPRHLLVCEKSNFGNHKSRHRHLVQTHYYNYRVSFLIPECGILSEELKNLVMNTGPYYFVK
  NLPLHELITPEFISTFIKKGSCYALTYNTHIDEDNTVALLPNGKLILSLDKDTYEETGLQGHPSQFSGRK
  IMKFIVSIDLMELSLNLDSKKYERISWSFKEKKPLKFDFLLAWHKTGSEESTMMSYFSKYQIQEHQPKVA
  LSTLRDLQCPVLQSSELEGTPEVSCRALELFDWLGAVFSNVDLNNEPNNFISTYCCPEPSTVVAKAYLCT
  ITGFILPEKICLLLEHLCHYFDEPKLAPWVTLSVQGFADSPVSWEKNEHGFRKGGEHLYNFVIFNNQDYW
  LQMAVGANDHCPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_006638
• ORF Size: 1089 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_006638.2
• RefSeq Size: 1174 bp
• RefSeq ORF: 1092 bp
• Locus ID: 10799
• UniProt ID: O75818
• Cytogenetics: 6p25.1
• MW: 41.7 kDa