Human PTGIS (NM_000961) AAV Particle

CAT#: RC211030A1V

AAV ORF Particles, serotype AAV-2, PTGIS (Myc-DDK-tagged)-Human prostaglandin I2 (prostacyclin) synthase (PTGIS), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: PTGIS
• Synonyms: CYP8; CYP8A1; PGIS; PTGI
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211030 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTGGGCCGCGCTCCTCGGCCTCCTGGCCGCACTGTTGCTGCTGCTGCTACTGAGCCGCCGCCGCA
  CGCGGCGACCTGGTGAGCCTCCCCTGGACCTGGGCAGCATCCCCTGGTTGGGGTATGCCTTGGACTTTGG
  AAAAGATGCTGCCAGCTTCCTCACGAGGATGAAGGAGAAGCACGGTGACATCTTTACTATACTGGTTGGG
  GGCAGGTATGTCACCGTTCTCCTGGACCCACACTCCTACGACGCGGTGGTGTGGGAGCCTCGCACCAGGC
  TCGACTTCCATGCCTATGCCATCTTCCTCATGGAGAGGATTTTTGATGTGCAGCTTCCACATTACAGCCC
  CAGTGATGAAAAGGCCAGGATGAAACTGACTCTTCTCCACAGAGAGCTCCAGGCACTCACAGAAGCCATG
  TATACCAACCTCCATGCAGTGCTGTTGGGCGATGCTACAGAAGCAGGCAGTGGCTGGCACGAGATGGGTC
  TCCTCGACTTCTCCTACAGCTTCCTGCTCAGAGCCGGCTACCTGACTCTTTACGGAATTGAGGCGCTGCC
  ACGCACCCATGAAAGCCAGGCCCAGGACCGCGTCCACTCAGCTGATGTCTTCCACACCTTTCGCCAGCTC
  GACCGGCTGCTCCCCAAACTGGCCCGTGGCTCCCTGTCAGTGGGGGACAAGGACCACATGTGCAGTGTCA
  AAAGTCGCCTGTGGAAGCTGCTATCCCCAGCCAGGCTGGCCAGGCGGGCCCACCGGAGCAAATGGCTGGA
  GAGTTACCTGCTGCACCTGGAGGAGATGGGTGTGTCAGAGGAGATGCAGGCACGGGCCCTGGTGCTGCAG
  CTGTGGGCCACACAGGGGAATATGGGTCCCGCTGCCTTCTGGCTCCTGCTCTTCCTTCTCAAGAATCCTG
  AAGCCCTGGCTGCTGTCCGCGGAGAGCTCGAGAGTATCCTTTGGCAAGCGGAGCAGCCTGTCTCGCAGAC
  GACCACTCTCCCACAGAAGGTTCTAGACAGCACACCTGTGCTTGATAGCGTGCTGAGTGAGAGCCTCAGG
  CTTACAGCTGCCCCCTTCATCACCCGCGAGGTTGTGGTGGACCTGGCCATGCCCATGGCAGACGGGCGAG
  AATTCAACCTGCGACGTGGTGACCGCCTCCTCCTCTTCCCCTTCCTGAGCCCCCAGAGAGACCCAGAAAT
  CTACACAGACCCAGAGGTATTTAAATACAACCGATTCCTGAACCCTGACGGATCAGAGAAGAAAGACTTT
  TACAAGGATGGGAAACGGCTGAAGAATTACAACATGCCCTGGGGGGCGGGGCACAATCACTGCCTGGGGA
  GGAGTTATGCGGTCAACAGCATCAAACAATTTGTGTTCCTTGTGCTGGTGCACTTGGACTTGGAGCTGAT
  CAACGCAGATGTGGAGATCCCTGAGTTTGACCTCAGCAGGTACGGCTTCGGTCTGATGCAGCCGGAACAC
  GACGTGCCCGTCCGCTACCGCATCCGCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211030 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRMKEKHGDIFTILVG
  GRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDVQLPHYSPSDEKARMKLTLLHRELQALTEAM
  YTNLHAVLLGDATEAGSGWHEMGLLDFSYSFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQL
  DRLLPKLARGSLSVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQ
  LWATQGNMGPAAFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPVLDSVLSESLR
  LTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFKYNRFLNPDGSEKKDF
  YKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLELINADVEIPEFDLSRYGFGLMQPEH
  DVPVRYRIRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_000961
• ORF Size: 1500 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_000961.3
• RefSeq Size: 5603 bp
• RefSeq ORF: 1503 bp
• Locus ID: 5740
• UniProt ID: Q16647
• Cytogenetics: 20q13.13
• MW: 57.1 kDa