Mouse Spp1 (NM_001204233) AAV Particle

CAT#: MR229068A1V

AAV ORF Particles, serotype AAV-2, Spp1 (myc-DDK-tagged) - Mouse secreted phosphoprotein 1 (Spp1), transcript variant 5, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Spp1
• Synonyms: 2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1; OP; Opn; Opnl; Ric; Spp-1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229068 representing NM_001204233
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGATTGGCAGTGATTTGCTTTTGCCTGTTTGGCATTGCCTCCTCCCTCCCGGTGAAAGTGACTGATT
  CTGGCAGCTCAGAGGAGAAGCTTTACAGCCTGCACCCAGATCCTATAGCCACATGGCTGGTGCCTGACCC
  ATCTCAGAAGCAGAATCTCCTTGCGCCACAGAATGCTGTGTCCTCTGAAGAAAAGGATGACTTTAAGCAA
  GAAACTCTTCCAAGCAATTCCAATGAAAGCCATGACCACATGGACGACGATGATGACGATGATGATGACG
  ATGGAGACCATGCAGAGAGCGAGGATTCTGTGGACTCGGATGAATCTGACGAATCTCACCATTCGGATGA
  GTCTGATGAGACCGTCACTGCTAGTACACAAGCAGACACTTTCACTCCAATCGTCCCTACAGTCGATGTC
  CCCAACGGCCGAGGTGATAGCTTGGCTTATGGACTGAGGTCAAAGTCTAGGAGTTTCCAGGTTTCTGATG
  AACAGTATCCTGATGCCACAGATGAGGACCTCACCTCTCACATGAAGAGCGGTGAGTCTAAGGAGTCCCT
  CGATGTCATCCCTGTTGCCCAGCTTCTGAGCATGCCCTCTGATCAGGACAACAACGGAAAGGGCAGCCAT
  GAGTCAAGTCAGCTGGATGAACCAAGTCTGGAAACACACAGACTTGAGCATTCCAAAGAGAGCCAGGAGA
  GTGCCGATCAGTCGGATGTGATCGATAGTCAAGCAAGTTCCAAAGCCAGCCTGGAACATCAGAGCCACAA
  GTTTCACAGCCACAAGGACAAGCTAGTCCTAGACCCTAAGAGTAAGGAAGATGATAGGTATCTGAAATTC
  CGAATTTCTCATGAATTAGAGAGTTCATCTTCTGAGGTCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229068 representing NM_001204233
  Red=Cloning site Green=Tags(s)
  
  MRLAVICFCLFGIASSLPVKVTDSGSSEEKLYSLHPDPIATWLVPDPSQKQNLLAPQNAVSSEEKDDFKQ
  ETLPSNSNESHDHMDDDDDDDDDDGDHAESEDSVDSDESDESHHSDESDETVTASTQADTFTPIVPTVDV
  PNGRGDSLAYGLRSKSRSFQVSDEQYPDATDEDLTSHMKSGESKESLDVIPVAQLLSMPSDQDNNGKGSH
  ESSQLDEPSLETHRLEHSKESQESADQSDVIDSQASSKASLEHQSHKFHSHKDKLVLDPKSKEDDRYLKF
  RISHELESSSSEVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_001204233
• ORF Size: 882 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_001204233.1, NP_001191162.1
• RefSeq Size: 1517 bp
• RefSeq ORF: 885 bp
• Locus ID: 20750
• UniProt ID: P10923
• Cytogenetics: 5 50.68 cM
• MW: 32.5 kDa