Mouse Gsk3a (NM_001031667) AAV Particle

CAT#: MR227461A1V

AAV ORF Particles, serotype AAV-2, Gsk3a (Myc-DDK-tagged) - Mouse glycogen synthase kinase 3 alpha (Gsk3a), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Gsk3a
• Synonyms: 2700086H06Rik
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227461 representing NM_001031667
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGCGGCGGGCCTTCGGGAGGCGGCCCTGGGGGCTCGGGCCGGGCGCGGACCAGCTCGTTCGCGG
  AGCCAGGAGGCGGAGGCGGAGGTGGTGGCGGCGGCCCCGGGGGCTCGGCCTCTGGCCCAGGAGGCACTGG
  CGGCGGGAAAGCGTCAGTCGGGGCTATGGGTGGGGGCGTGGGAGCCTCGAGCTCCGGGGGTGGCCCCAGC
  GGCAGCGGCGGAGGAGGCAGCGGTGGCCCCGGCGCGGGCACTAGCTTCCCGCCGCCGGGAGTGAAGCTGG
  GCCGTGACAGCGGGAAGGTGACCACAGTGGTAGCCACTGTAGGCCAAGGCCCAGAGCGTTCCCAAGAAGT
  GGCTTACACTGACATCAAAGTGATTGGTAATGGCTCATTCGGAGTAGTATACCAGGCACGGCTGGCAGAG
  ACGAGGGAACTGGTGGCCATCAAGAAGGTTCTTCAGGACAAAAGGTTCAAGAACCGAGAACTGCAGATTA
  TGCGTAAGCTGGACCACTGCAATATTGTGAGGCTGCGGTACTTTTTCTACTCCAGTGGGGAGAAGAAGGA
  CGAGCTGTATTTGAATCTGGTGCTGGAGTATGTGCCCGAGACGGTGTACCGAGTGGCCCGCCACTTCACC
  AAGGCCAAGCTGATCACCCCTATCATCTACATCAAGGTGTACATGTACCAGCTCTTCCGGAGCTTGGCCT
  ACATCCACTCCCAAGGTGTGTGTCACCGTGACATCAAGCCCCAGAATTTGCTTGTGGACCCTGACACTGC
  TGTCCTCAAGCTCTGCGATTTTGGCAGTGCAAAGCAGCTGGTTCGGGGGGAGCCCAATGTGTCCTACATC
  TGCTCTCGGTACTACCGTGCTCCAGAACTCATCTTTGGAGCCACAGATTACACCTCGTCCATCGATGTGT
  GGTCGGCTGGCTGTGTACTTGCTGAGCTACTTCTCGGCCAGCCCATCTTCCCTGGGGACAGTGGGGTGGA
  CCAGCTTGTGGAGATCATCAAGGTACTAGGAACGCCAACCAGGGAACAAATCCGAGAGATGAACCCTAAC
  TATACGGAGTTCAAGTTCCCCCAGATCAAAGCTCACCCTTGGACAAAGGTGTTCAAATCTTCAAAGACAC
  CACCTGAGGCCATTGCACTCTGCTCTAGCCTACTGGAGTACACGCCATCCTCAAGGCTCTCCCCACTCGA
  GGCTTGTGCCCACAGCTTCTTCGATGAACTGCGGAGACTCGGAGCCCAGCTCCCCAACGACCGCCCGCTT
  CCCCCCCTGTTCAACTTCAGTCCTGGTGAACTGTCCATCCAACCATCTCTCAATGCCATTCTCATCCCTC
  CTCACTTGAGGTCCCCAGCAGGCCCTGCTTCTCCCCTCACCACTTCCTACAACCCATCCTCACAAGCTTT
  AACTGAAGCTCAGACTGGCCAAGATTGGCAGCCATCTGATGCCACAACTGCTACCCTCGCTAGCTCTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227461 representing NM_001031667
  Red=Cloning site Green=Tags(s)
  
  MSGGGPSGGGPGGSGRARTSSFAEPGGGGGGGGGGPGGSASGPGGTGGGKASVGAMGGGVGASSSGGGPS
  GSGGGGSGGPGAGTSFPPPGVKLGRDSGKVTTVVATVGQGPERSQEVAYTDIKVIGNGSFGVVYQARLAE
  TRELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDELYLNLVLEYVPETVYRVARHFT
  KAKLITPIIYIKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKLCDFGSAKQLVRGEPNVSYI
  CSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPN
  YTEFKFPQIKAHPWTKVFKSSKTPPEAIALCSSLLEYTPSSRLSPLEACAHSFFDELRRLGAQLPNDRPL
  PPLFNFSPGELSIQPSLNAILIPPHLRSPAGPASPLTTSYNPSSQALTEAQTGQDWQPSDATTATLASSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_001031667
• ORF Size: 1470 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_001031667.1, NP_001026837.1
• RefSeq Size: 2276 bp
• RefSeq ORF: 1473 bp
• Locus ID: 606496
• UniProt ID: Q2NL51
• Cytogenetics: 7 A3
• MW: 52.1 kDa