Mouse Crkl (NM_007764) AAV Particle

CAT#: MR225132A1V

AAV ORF Particles, serotype AAV-2, Crkl (Myc-DDK-tagged) - Mouse v-crk sarcoma virus CT10 oncogene homolog (avian)-like (Crkl), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Crkl
• Synonyms: 1110025F07Rik; AA589403; AI325100; Cr; Crkol; snoop
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225132 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCCGCCAGGTTTGATTCTTCAGACCGCTCTGCCTGGTACATGGGGCCAGTGACTCGCCAGGAGG
  CGCAGACTCGTCTCCAAGGCCAGCGCCATGGCATGTTCCTAGTCCGGGACTCATCTACCTGCCCCGGGGA
  CTATGTACTGTCCGTGTCCGAGAACTCGCGTGTCTCGCACTACATCATCAACTCCCTGCCCAACCGCCGC
  TTTAAGATCGGGGACCAGGAGTTTGACCATTTGCCGGCCTTGTTAGAGTTCTACAAGATCCACTACCTGG
  ACACTACCACCTTAATCGAACCGGCGCCCAGGTACCCAAGCCCACCAGTGGGTTCTGTCTCAGCACCCAA
  CTTACCTACAGCAGAAGAAAATCTGGAATATGTACGGACCCTTTATGATTTTCCTGGGAATGATGCTGAA
  GACCTACCCTTTAAAAAGGGCGAGCTTCTAGTGATAATAGAAAAGCCTGAAGAGCAGTGGTGGAGTGCCC
  GCAACAAGGACGGCCGGGTTGGGATGATTCCTGTCCCTTACGTTGAAAAGCTTGTGAGGTCTTCGCCACA
  TGGAAAGCATGGAAATAGGAATTCTAACAGTTATGGCATCCCAGAACCTGCTCATGCGTATGCTCAACCT
  CAGACCACAACTCCTCTACCTACAGTTGCCAGTACTCCTGGGGCAGCGATCAACCCTTTGCCATCCACAC
  AGAATGGACCTGTCTTTGCAAAAGCAATCCAGAAGAGAGTACCTTGTGCTTATGACAAGACTGCCTTGGC
  ATTGGAGGTTGGTGACATTGTGAAAGTCACAAGGATGAATATCAATGGCCAATGGGAAGGCGAGGTGAAT
  GGGCGCAAGGGGCTTTTCCCCTTCACACATGTTAAAATCTTTGACCCTCAGAACCCCGATGACAACGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225132 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSARFDSSDRSAWYMGPVTRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRR
  FKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPSPPVGSVSAPNLPTAEENLEYVRTLYDFPGNDAE
  DLPFKKGELLVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQP
  QTTTPLPTVASTPGAAINPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVN
  GRKGLFPFTHVKIFDPQNPDDNE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_007764
• ORF Size: 912 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_007764.4, NP_031790.2
• RefSeq Size: 5050 bp
• RefSeq ORF: 912 bp
• Locus ID: 12929
• UniProt ID: P47941
• Cytogenetics: 16 A3
• MW: 33.8 kDa