Mouse Alg1 (NM_145362) AAV Particle

CAT#: MR215774A1V

AAV ORF Particles, serotype AAV-2, Alg1 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (Alg1), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Alg1
• Synonyms: HMAT1; HMT1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215774 representing NM_145362
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGTCCTGCGTGGCGCTGCTGGTTCTGGCGCTGCTGTTGCTGGTGCTGCTGCTGGGCTTGTGGA
  AGCGTGGGCGGCAAACGGGCAGGGCCCGGCACATGGTCGTCGTGGTGCTGGGCGACGTGGGCCGCAGCCC
  CCGCATGCAGTACCACGCGCTGTCATTGGCCCAGAGCGGCTTTTCGGTGACGCTGCTGGGGTTCTACAAC
  TCCAAACCTCGAGATGAACTCTTGCAGAATGATAGGATTCGGATTGTGAAGTTGACAGATCTGCGGGGCC
  TGGGAGCTGGGCCCCGAATTCTCCAGTATGGAGTCAAGGTTGTATTTCAGGCAGTGTACTTGCTGTGGAA
  GATGATGCGCATGGACCCTGCAGCCTACATCTTCCTCCAGAACCCTCCAGGTCTGCCTGCTATTGCCGTC
  TGCTGGTTTGTGGGCTGCATTTGTGGGAGCAAATTGGTCATCGACTGGCACAACTATGGCTACTCCATCA
  TGGGCTTGGTCCATGGCCCCCGCCACCCCATTGTGCTGCTGGCCAAATGGTATGAGAAGTTCTTCGGGCG
  CCTGTCCCACCTGAACCTGTGTGTGACCAATGCTATGCGGGAGGACCTGGCAGAGAACTGGTGTGTCAGG
  GCTGTGACGCTCTACGACAAGCCAGCATCTTTCTTTAAGGAGACACCCCTGGACCTGCAGCATGAACTCT
  TTATGAAGCTGAGCCACACGTACTCTCCTTTCCAGAGCTGCTCAGATCCCTCACATCCTGACACAGAGAG
  GTCGGCCTTCACTGAGAGGGATTGTCAGAGCGGGGTTGTGAGGCGTCTGCATGGGCGGCCAGCACTGCTC
  GTGAGCAGCACAAGCTGGACAGAGGACGAGGACTTCTCCATCCTGCTTCGAGCATTAGAAAAGTTTGAGC
  AACAAGCACTCACTGGAGACAGCCTCCCTTCCCTCGTCTGTGTGATAACAGGGAAGGGACCGCTCAGAGA
  GCACTACCGCCACCTCATCAGCCAGAAGCACCTTCAGCACGTCCGGTTCTGCACCCCATGGCTGGAAGCC
  GAGGACTACCCACTGCTTCTAGGGTCAGCGGATCTGGGTGTCTGTCTACACATGTCCTCCAGTGGTCTGG
  ACCTGCCCATGAAGGTGGTGGACATGTTTGGGTGCCACTTGCCTGTGTGTGCCGTGAACTTCAAGTGTCT
  GCACGAGCTAGTAAGACATGGAGAGAATGGTCTGGTCTTCAAGGATGCTGAGGAGCTGGCAGCTCAGCTG
  CAGATGCTTTTCTCAAAGTTCCCAGACCCTGCTGGAAAGCTAAGCCAGTTCCGGAAGAAACTGCAGGAGT
  CGGGGCAGCAACGCTGGGATGAGAGCTGGCAGCACACTGTGCTCCCTCTGCTCGCTCACTCACAGATGAC
  CCCCAGGCCCCATCCACCCTGTGGCCACCCTTCCTGCCGGGGCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215774 representing NM_145362
  Red=Cloning site Green=Tags(s)
  
  MAASCVALLVLALLLLVLLLGLWKRGRQTGRARHMVVVVLGDVGRSPRMQYHALSLAQSGFSVTLLGFYN
  SKPRDELLQNDRIRIVKLTDLRGLGAGPRILQYGVKVVFQAVYLLWKMMRMDPAAYIFLQNPPGLPAIAV
  CWFVGCICGSKLVIDWHNYGYSIMGLVHGPRHPIVLLAKWYEKFFGRLSHLNLCVTNAMREDLAENWCVR
  AVTLYDKPASFFKETPLDLQHELFMKLSHTYSPFQSCSDPSHPDTERSAFTERDCQSGVVRRLHGRPALL
  VSSTSWTEDEDFSILLRALEKFEQQALTGDSLPSLVCVITGKGPLREHYRHLISQKHLQHVRFCTPWLEA
  EDYPLLLGSADLGVCLHMSSSGLDLPMKVVDMFGCHLPVCAVNFKCLHELVRHGENGLVFKDAEELAAQL
  QMLFSKFPDPAGKLSQFRKKLQESGQQRWDESWQHTVLPLLAHSQMTPRPHPPCGHPSCRGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_145362
• ORF Size: 1446 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_145362.2, NP_663337.2
• RefSeq Size: 1772 bp
• RefSeq ORF: 1449 bp
• Locus ID: 208211
• UniProt ID: Q921Q3
• Cytogenetics: 16 A1
• MW: 54.9 kDa