Mouse Lipa (NM_001111100) AAV Particle

CAT#: MR206231A1V

AAV ORF Particles, serotype AAV-2, Lipa (Myc-DDK-tagged) - Mouse lysosomal acid lipase A (Lipa), transcript variant 2, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Lipa
• Synonyms: AA960673; Lal; Lip-1; Lip1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206231 representing NM_001111100
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACTCCAGGGCCTGGTGTTCGTTTTCACCATTGGGATCCTGCTTTCTCGGGTGCCCACGGGAACTG
  TATCAGCTGTGGATCCTGAAGTCAACATGAATGTGACCGAGATAATCATGCGCTGGGGATATCCTGGTGA
  GGAACACTCGGTCCTGACAGGAGATGGCTATATTCTGAGTATTCACCGAATCCCTCGTGGGCGGAAGAAC
  CATTTTGGCAAAGGTCCCAGACCAGTTGTGTATCTTCAGCACGGCTTGCTGGCAGATTCTAGTAACTGGG
  TCACAAACATTGACAACAGCAGCTTGGGCTTCCTCCTGGCGGATGCTGGCTTTGATGTGTGGATGGGAAA
  CAGCAGAGGAAACACCTGGTCTCTGAAGCACAAGACTCTTTCAGTTTCTCAGGATGAGTTCTGGGCCTTC
  AGTTTTGATGAGATGGCAAAATATGACCTACCTGCTTCAATTAACTACATTTTGAATAAAACTGGCCAAG
  AACAAATCTATTATGTGGGTCATTCTCAAGGCTGCACCATAGGTTTCATAGCTTTTTCACAGATGCCTGA
  GTTGGCAAAGAAGATTAAAATGTTTTTAGTCTTGGCTCCCGTGTTGTCTCTCAATTTTGCTTCAGGCCCG
  CTACTTCAATTGGGACGCTTGCCAGACCCTCTTCTCAAGGACATGTTTGGACAGAAGCAATTTCTCCCTC
  AGAGTGCGATGCTGAAGTGGCTGAGCATTCACGTTTGCACCCACGTCATCATGAAGGAGCTTTGCGCAAA
  CGTCTTCTTCCTGCTATGTGGCTTCAATGAGAAGAATTTAAATATGTCTAGAGTGGATGTGTATACAACA
  CACTGCCCTGCGGGAACTTCTGTGCAAAACATGTTACACTGGGGTCAGGTTTTTAAATACCGTAAGCTTC
  AAGCTTTTGACTGGGGAAGCAGTGAAAAGAATTATTTTCATTACAACCAGAGTTTTCCTCCCTCGTACAA
  CATAAAAAACATGCGTCTACCCACTGCTTTGTGGAGCGGGGGCCGGGACTGGCTGGCAGACATCAATGAC
  ATCACTATCTTACTGACTCAAATCCCCAAGTTGGTGTATCACAAGAACATTCCTGAGTGGGACCATCTGG
  ATTTTATCTGGGGTTTGGATGCCCCCTGGAAACTGTATGATGAAATAATCAGTCTAATGAAGAAATACCA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206231 representing NM_001111100
  Red=Cloning site Green=Tags(s)
  
  MQLQGLVFVFTIGILLSRVPTGTVSAVDPEVNMNVTEIIMRWGYPGEEHSVLTGDGYILSIHRIPRGRKN
  HFGKGPRPVVYLQHGLLADSSNWVTNIDNSSLGFLLADAGFDVWMGNSRGNTWSLKHKTLSVSQDEFWAF
  SFDEMAKYDLPASINYILNKTGQEQIYYVGHSQGCTIGFIAFSQMPELAKKIKMFLVLAPVLSLNFASGP
  LLQLGRLPDPLLKDMFGQKQFLPQSAMLKWLSIHVCTHVIMKELCANVFFLLCGFNEKNLNMSRVDVYTT
  HCPAGTSVQNMLHWGQVFKYRKLQAFDWGSSEKNYFHYNQSFPPSYNIKNMRLPTALWSGGRDWLADIND
  ITILLTQIPKLVYHKNIPEWDHLDFIWGLDAPWKLYDEIISLMKKYQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_001111100
• ORF Size: 1191 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_001111100.1, NP_001104570.1
• RefSeq Size: 2987 bp
• RefSeq ORF: 1194 bp
• Locus ID: 16889
• UniProt ID: Q9Z0M5
• Cytogenetics: 19 C1
• MW: 45.8 kDa