DCAMKL1 (DCLK1) (NM_004734) Human 3' UTR Clone

CAT#: SC222414

3' UTR clone of doublecortin-like kinase 1 (DCLK1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DCLK1
• Synonyms: CL1; CLICK1; DCAMKL1; DCDC3A; DCLK
• ACCN: NM_004734
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC222414 3’UTR clone of NM_004734
  The sequence shown below is from the reference sequence of NM_004734. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TTTTCCGACGAAGACGCAACCAGGATGTGAGGAGCCGGTACAAGGCGCAGCCAGCTCCTCCCGAACTCA
  ACTCGGAATCGGAAGACTACTCCCCAAGCTCCTCCGAGACTGTTCGCTCCCCTAACTCGCCCTTTTAAT
  AAGACCCTTTTACTCAAAGTCCTAGCTTAACCCTTTGAGACTCTGAGATTTTTTTCCCCCAAATTTGTG
  TAAAACAGTTTCATCTGATCTATCTAGCGCTCAATGCTTGAATGGCAGAACTGAAAGTGTTTTCAGGTA
  TCTTTGTAGCGGTTTCCCTTTACTGAATAAGATGACACGTGGTGATTGTGAAGATGGTAATTTGCTGCT
  AATAGAGTCCTCAAAGGGTTAAGGCCAATTTGCAATTTTTTTTTAAACTTAGAAGCAATGAATGTTTTC
  ATCAGTCAAGCTAGGATCTGCAGTATGTAATATAGCACTTGTTAACCCTCTGAGTGCATAGAATTTTAT
  TGAGAATTCTTGTTTGGGAATTTTTCAGGCCTTTGGATGTATACACACATGTTTCTTGATTTTACTGCA
  GATCAAGGGGTGTTGTTAGATGCTGAAATGTCCAGAAAAGAAGGACATTTAGAATGATATCTTGTTTGT
  CCTTTTCTGTGGGTTTAGAACGTGGCAGGTTTATAACTTCGACACACGCACGGTTCTTTCTTCTTCACA
  ATCCTATTCAGAAACAGATTTTTTTTTTCATTAGAGATATGACTGTCAGTTGCAGTGAGTTCTGCATCC
  CAAGTGGAGGGAATTGGGTTTGTGGCAAAGAGCTTGACCCAGGAAATAGATGGTGCCCCCCAAATTGTC
  TCCACATGAAGATGTACTGATGACGCCCCAGAAATGCTGCTTCCATATCAGCTGCTGCTAGCGCCAGCG
  CAGACTCTCAGGGAGTCACCACAGCTTGTCTTGTGCTTGGTGAGTGAGGGTCTCTCTACTCAGTGTCAG
  ACATCTACAGGAAAGAAACAACTGGTGGAAAAGAGCAATAAATTGCCCGGTGCTCTGCAGGGCTGGAAT
  TTCAAACAGAAAGAGGGAATAAGATCCTGTGATTTTTCTCACCTGCTTTTCCACGCACTGTGGTCATCA
  CTGTGCAATCTACATCTAGTATGAAATCCACACATAGGAGAGCTGGGGCACAAGGGGACTGGAGGCAGT
  TGCTTTGCAAGATGGCTGAGGAGAAAGCACACTGGGAACACAATCCAGAATGTTCTAACAATAAGTTTT
  CAGTGAATAAACCACTGGCAAGACAATTCCATGTGCACCTTTAGGTTACCTATATAGTCTCCTAGGAAG
  ATCAGGATGAAAGACCTAGATGATACCCCTGAGGATAAAACCTCCATCCCCTAAAATGATTTTTTTTAA
  ATACCACTGTCTTTAGCTGTCCAGGAGGTCAGAGTGTTTTTTCTGTCTTTGGGCCAAGTCCTGTCTGAG
  ACCTGTATTTTCACTCTTGTTACCAAATCTATCTCCCTAGTGCAGTGTCTCCAGGCCTGAGTTTCTTCT
  GGAACAGATTCCATTTTAGAATGGGGATTCACAGGTTCTGTGCATCACCACAGTGCTCAGAGAGGATTC
  TCCTGGGGTGTCTTAGAGGCAGGTGCCCAACTCAAATGTATTCCCAAGGTTTGCTGGGCTCTGGGATCC
  ACGAGACAACCAGAGAGGGATATCTCATGAAATTTGCATCTGGTGGCTGAACAGTACCTATGTTCTCTG
  TTTTGAATATACTTTAATACCTGAGAGTCTTAAAATTTGTGAACAACGTTTCTATAGTCCTTTATTTTC
  AAATGCACATTGATCTTCACTTGCTGCATTTTTACTCTTCAACCCTGAAACTATGGTCTACATTAATAT
  GGATTTTTAAATCACATGTCATTACTTTTGCAACACCATCACCAAAATTTTTTGCTCTTTTACATTTAG
  GTTCATCTCTGTGGTCTGTGTTGTCCTGACATGTAAAAAGCATATCGTTTATTGAGGTTTTTTTCCCC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004734.5
• Summary: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.[provided by RefSeq, Sep 2010]
• Locus ID: 9201
• MW: 76.2