PLAG1 (NM_001114634) Human 3' UTR Clone

CAT#: SC222303

3' UTR clone of pleiomorphic adenoma gene 1 (PLAG1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PLAG1
• Synonyms: PSA; SGPA; SRS4; ZNF912
• ACCN: NM_001114634
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC222303 3’UTR clone of NM_001114634
  The sequence shown below is from the reference sequence of NM_001114634. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CTCCCACGTTTCCATCAAGCTTTTCAGTAGGATTCTGGGACATGGATTCATTACAGAAATGTATGTGTA
  GCTGTGCCCTAGATGACCATTTTTATTTTAGTGCCTACTTTAAAACAGTATAAAAATTTCTGCTTTTGT
  ATAATACAAATTTTCATTAAGCCAGTATAAAATAGAAACTAGCTTTTAAACTGAGCTTTGGAACCATTT
  GTGTTCAGTTAAGTTTACCTGGGTATTTTGTCCTGATTCACTGCCAATTGTCACATTTTAAGACTTTTT
  TTTTTCCATATAGGAAAGCCATTATTAGTAGTAAACTTTTACAAATCCCATTTTCAAATTACTTTTAGA
  TCTTAAAATTTTCATTTTTGTCTAATAACAGTGGCTCTACCTTTTGACATCTGGCTCATTAAAAAATTT
  AGCAATAGAATGTAAATTGTATAAAAAGTTTGTGAATAACTCAAGGGTTTAAATTTTCTTACTAGCTTC
  TAAATGGATTAATAATCAAGTGCTTCAAATGAATTAAGAGTCCAGTTTCGGAAGATAATAAATGTTTGT
  TAGATACACCATAATTTCAGATCAGTATATTCTGAAGACTCCCTGTTGTCTGGCTAAAATATTTGCCAT
  CTTTATTATGAGCCTTTAAGGAAAACAAACCCTAAACACAAAGCATCAGTATTTATAGCAAAAAGAGAC
  TCTGTTAGGTGACATGGCATTTCGTGTCACTTAATAGTTGGCCCTAAATTAGTACACAGGATATTTTGT
  CGTGTTTCATCCTTCTTAACATGCTATCTTTTCATTTAATAATAGTAATAGTGTATGGCATTGGGGTCT
  TCAGAGTCGATATATAGGTAGATCTCTTTAGTCTTTTCCACCTTTCACATCCAAGGGGTGGGTCAAGTG
  CAGCCAGCAATTTATTTTCATTGTTGGCCCACGGTTAGTCCATAATCTAGAGCCATTGTGGAACTGCAG
  CCATGAGGTGTGTTTATCCCACAGTGGATTGACTCAGCCTCTGTGGGTGACAGACTTCTAAGCAGGAAG
  ATAGACGTGAAGCACATGGTTACATTTGGGAACTTGTGTAGGGATCATGGCCCCTGTAGCCAGGGTTAA
  AAACTGGACTTTTTAGAAGTAAAGTAAAAGCATATCGCTTATATCATTTCTTGCTGAATTTGATATGTT
  TTTCTTTCCCTTAAGAATCAAAAGCAGAAAACAAAAACAACAGTCCTACTCCGATGTTATCTTTCTGAT
  TCAATGTGAATCCATCTTTCCTTGCAATATTTTGGATGGAGAATTTGAAGTTAAATGCATTAGAAAACT
  ACCTGATGAACTACCACAAAGTTTTAAGTGACTAGAAATATATACAGTAAAATCCCACTTTCATGCATC
  TCTGGGAAATGATAGGAGTATTGCAAATAAGTTGAGTTTGTAGAGGGTAACAAAGTAAAGTAAAACAAA
  CCTATCTTGGTTAACATGAAAATAACAATTGAGAATATATTATATTCACTGAATAATTATAGGCTTTTC
  CTCACATTAGACAACCAACATAATCTTCTTAAAGGTCTAATTAATATATTTTTCTAAGGGTCAGTTGGG
  ACATTAACCTAAGAAACATATCTATTAAGCACTTGTTAACACCTTATTTTAGGACCCTTTCCGTTGGGG
  ATGGGGGCAAGGGTGGGAGGTTTTTAGAAGAGTATATATCTCTTTAAAAAAAAACAGAAAGAAAAATAT
  TTCTGAGCACTCATTAGCCCTATATGGAAACTTCTTTCCTTTTTGTAGGGCCAGTTATCACTGCAGATT
  GCAATGTTTACCAAGAATTTCTAAAAATGAGTGCAGATTACTGAATATAATACATTATTTAAAATATTT
  GGGAGTAGTATAATTTGTGGAGAAATGTAAATTGTAATAATGTAAATGGGGGCTTCAATATATATATAT
  AATACACACACACACACACATGCACACATACCGCACTTCATAGAATCAAAGTTGCTCTCTGAAGGAGC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001114634.2
• Summary: Pleomorphic adenoma gene 1 encodes a zinc finger protein with 2 putative nuclear localization signals. PLAG1, which is developmentally regulated, has been shown to be consistently rearranged in pleomorphic adenomas of the salivary glands. PLAG1 is activated by the reciprocal chromosomal translocations involving 8q12 in a subset of salivary gland pleomorphic adenomas. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 5324
• MW: 76.9