LSS (NM_001145437) Human 3' UTR Clone

CAT#: SC217287

3' UTR clone of lanosterol synthase (23-oxidosqualene-lanosterol cyclase) (LSS) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: LSS
• Synonyms: APMR4; CTRCT44; HYPT14; OSC
• ACCN: NM_001145437
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC217287 3’UTR clone of NM_001145437
  The sequence shown below is from the reference sequence of NM_001145437. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCTGAGAGAGCCCTTGCTGGCCACCCCTGAGAACATGCCTACCTGCTGGGTGCCGTCTGTGCGTTCCAG
  TGAGGCCAAGGGGTCCTGGCCGGGTTGGGGAGCCCTCCCATAACCCTGTCTTGGGCTCCAACCCCTCAA
  CCTCTATCTCATAGATGTGAATCTGGGGGCCAGGCTGGAGGCAGGGATGGGGACAGGGTGGGTGGCTTA
  GACTCTTGATTTTTACTGTAGGTTCATTTCTGAAAGTAGCTTGTCGGGCTTGGGTGAGGAAGGGGGCAC
  AGGAGCCGTGACCCCTGAGGAGGCACAGCGCCTTCTGCCACCTCTGGGCACGGCCTCAAGGTAGTGAGG
  CTAGGAGGTTTTTTCTGACCAATAGCTGAGTTCTTGGGAGAGGAGCAGCTGTGCCTGTGTGATTCCTTA
  GTGTCGAGTGGGCTCTGGGCTGGGGTCGGCCCTGGGCAGGCTTCTCCTGCACCTTTTGTCTGCTGGGCT
  GAGGGACACGAGGGCAACCCTGTGACAATGGCAGGTAGTGTGCATCCGTGAATAGCCCAGTGCGGGGGT
  TGCTCATGGAGCATCCTGAGGCCGTGCAGCAGGGAGCCCCATGCCCCTGGGTCGTGAGCTTGCCTGCGT
  ATGGGGTGGTGTCATGGAGCCTCATGCCCCTGGGTCGTGAGCTCGCCTGAGTATGGGGTGGTGTCATGG
  AGCCGCATACCCCTGGGTTGTGAGCTCGCCTGCATATGCAGGGTCTGTCATGGAACATCCCAAGTCTGT
  GCAGCAGGGAGCCCCATGCCCCTGGGACATGAACCCACCTGCGTGGAATGCTGTTTGTGAGGTGTCTAC
  AGGGTTTATAGTAGTCTTGTGGACACAGAAATGCACAGGGGACACTTACGGACACAGAAATGCACAGGG
  GAGGCCGAGCATAACCAGGGGTGAGGGGCAGGCAGCAGTTGTAGTTACTGCCGCGGGGCACTGCTATGT
  GCAGGGACAGCCAGCACCCAGCCCATCACCACTCCCTGGGCTGGCTGGCAGGTATGGCACCCTGGGAGC
  CCGGCATATACCCAGGGCACCCCTACGGCTGCCGCCAGTCTCATGCCCAGGTGGGTGCTCTGGGCTGGA
  GCGAGGGCCAGGTTTTGGGCCGAGGCTTCCCCAGGCAATCCTGTGAGCTCCCTTCTAGCCTCTGACCCA
  GTCTGGTCTGGCTTGCATGGATGTAGGGCTTGGGGTGGGAAGTTCAGGTCCTGGCTTTGCCTTTGCCTG
  ATGTGGATGAGCAGCTCACATGCTCAGGGCCACCTGAGACTGTCACTGCTCTCCCCTGGCTACTGGGAG
  GAGTCACTGAGAGCTTCGTTACCCCTGCTGCCTTGCCCAGGGCACACCCTATACCTCCTTATCTGCTCT
  TCCCCTCCCTGCCGCCTTCTGGGCAGGTAGCAGTCCCTGGCCTCTCCCCCTGGCTGATCACTCTCCCTC
  AGGCAGTGGAGATCTGCGTCTGGACACCCTCAGATCCTGTCATTGCCTGCCCAGAGTCCTTCAGGGGCA
  CCCCTCTGCCTTGGTGTGCAGTCCAGGGCTCTCACCCAGGTGCCGCACCCTCTGGGGTCTTCTGTCCAG
  CTCCCTTGCCCCATGTGCTGTCACTGACTCTCCTTGGGACTCGCCTGCCTGCTCAGAGCCCTGCAGGGC
  TTGGTCAGCTGCCTGTTCAGTGTCAACACTTCCCTGCACATCTTAAAACTGGGCTTTATTTTCGCTGAA
  GGAACTGTGTTGGGACCCTTGACATCTGTCAGGTTTGCACATGCTGTTTTTTTTTCTCAGCCCACGTGT
  TCTCCCCCACGTGGGGTAGCAGCAGGACAGACAGTGAATCACAGAGTCTGCCCTGAGCAGAGGCTGCTG
  TCCCTGGGACTCCTAGCCATGGTCAGACTGTACAAAACGGTTTTCCAGAAATGAAATGTAAATCCATTT
  TTATACTGAAAATGTTACTGAAAGTCACTTTTATGAGCATCTGCCTTAATAAACAGACATTGATTCCC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001145437.2
• Summary: The protein encoded by this gene catalyzes the conversion of (S)-2,3 oxidosqualene to lanosterol. The encoded protein is a member of the terpene cyclase/mutase family and catalyzes the first step in the biosynthesis of cholesterol, steroid hormones, and vitamin D. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2009]
• Locus ID: 4047
• MW: 70.8