GRP78 (HSPA5) (NM_005347) Human 3' UTR Clone

CAT#: SC216266

3' UTR clone of heat shock 70kDa protein 5 (glucose-regulated protein 78kDa) (HSPA5) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GRP78
• Synonyms: BIP; GRP78; HEL-S-89n
• ACCN: NM_005347
• Insert Size: 1754 bp
• Sequence Data:
  Insert Sequence

  >SC216266 3' UTR clone of NM_005347
  The sequence shown below is from the reference sequence of NM_005347. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGGTGAAGAGGATACAGCAGAAAAAGATGAGTTGTAGACACTGATCTGCTAGTGCTGTAATATTGTAAA
  TACTGGACTCAGGAACTTTTGTTAGGAAAAAATTGAAAGAACTTAAGTCTCGAATGTAATTGGAATCTTC
  ACCTCAGAGTGGAGTTGAAACTGCTATAGCCTAAGCGGCTGTTTACTGCTTTTCATTAGCAGTTGCTCAC
  ATGTCTTTGGGTGGGGGGGAGAAGAAGAATTGGCCATCTTAAAAAGCGGGTAAAAAACCTGGGTTAGGGT
  GTGTGTTCACCTTCAAAATGTTCTATTTAACAACTGGGTCATGTGCATCTGGTGTAGGAAGTTTTTTCTA
  CCATAAGTGACACCAATAAATGTTTGTTATTTACACTGGTCTAATGTTTGTGAGAAGCTTCTAATTAGAT
  CAATTACTTATTTTAGGAAATTTAAGACTAGATACTCGTGTGTGGGGTGAGGGGAGGGAGTATTTGGTAT
  GTTGGGATAAGGAAACACTTCTATTTAATGCTTCCAGGGATTTTTTTTTTTTTTTTTAACCCTCCTGGGC
  CCAAGTGATCCTTCCACCTCAGTCTCCCAGCTAATTGAGACCACAGGCTTGTTACCACCATGCTCGGCTT
  TTGCATTAATCTAAGAAAAGGGGAGAGAAGTTAATCCACATCTTTACTCAGGCAAGGGGCATTTCACAGT
  GCCCAAGAGTGGGGTTTTCTTGAACATACTTGGTTTCCTATTTCCCCTTATCTTTCTAAAACTGCCTTTC
  TGGTGGCTTTTTTTAAAATTATTACTAATGATGCTTTTATAGCTGCTTGGATTCTCTGAGAAATGATGGG
  GAGTGAGTGATCACTGGTATTAACTTTATACACTTGGATTTCATTTGTAACTTTAGGATGTAAAGGTATA
  TTGTGAACCCTAGCTGTGTCAGAATCTCCATCCCTGAAATTTCTCATTAGTGGTACTGGGGTGGGATCTT
  GGATGGTGACATTGAAACTACACTAAATCCCCTCACTATGAATGGGTTGTTAAAGGCAATGGTTTGTGTC
  AAAACTGGTTTAGGATTACTTAGATTGTGTTCCTGAAGAAAAGAGTCCAGGTAAATGGTATGATCAATAA
  AGGACAGGCTGGTGCTAACATAAAATCCAATATTGTAATCCTAGCACTTTGGGAGGCCAAGGCGGGTGGA
  TCACAAGGTCAAGAGATAGAGACCATCTTTGCCAACATGGTGAAACTCCATCTCTACTGAAAATACAAAA
  ATTAGCTGGGCGTGGTAGTGCAAGCTGAAGGCTGAGGCAGGAGAATCACTCGAACCCGGGAGGCAGAGGT
  TGCAGTGAGCCGAGATCACACCACTGTACTCCAGCCCGGCACTCCAGCCTGGCGACAAGAGTGAGACTCC
  ACCTCAAAAAAAAAAAAAAGAATCCAATACTGCCCAAGGATAGGTATTTTATAGATGGGCAACTGGCTGA
  AAGGTTAATTCTCTAGGGCTAGTAGAACTGGATCCCAACACCAAACTCTTAATTAGACCTAGGCCTCAGC
  TGCACTGCCCGAAAAGCATTTGGGCAGACCCTGAGCAGAATACTGGTCTCAGGCCAAGCCCAATACAGCC
  ATTAAAGATGACCTACAGTGCTGTGTACCCTGGGGCAATAGGGTTAAATGGTAGTTAGCAACTAGGGCTA
  GTCTTCCCTTACCTCAAAGGCTCTCACTACCGTGGACCACCTAGTCTGTAACTCTTTCTGAGGAGCTGTT
  ACTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005347.3
• Summary: The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers. [provided by RefSeq, Jul 2020]
• Locus ID: 3309