Chimaerin 2 (CHN2) (NM_001039936) Human 3' UTR Clone

CAT#: SC215552

3' UTR clone of chimerin (chimaerin) 2 (CHN2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CHN2
• Synonyms: ARHGAP3; BCH; CHN2-3; RHOGAP3
• ACCN: NM_001039936
• Insert Size: 1623 bp
• Sequence Data:
  Insert Sequence

  >SC215552 3’UTR clone of NM_001039936
  The sequence shown below is from the reference sequence of NM_001039936. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TTAATAGAAAACGAAGACGTTTTATTCTAATCCATCAGGGAAATGAGCTGAATGGCCCCAGCACCATCC
  AAGTTGACACAGCTAAAGGAATAAAAACATTTCTTACCACTTGATTTGTTTTCCAAGCAAGTGCTAGAA
  TTTCCTGGACTGCAGAGGATCGCTGAGTGGGGTACTGTGTCTCATAGACATGCGCCACCTCCACGTGAG
  AACAAGGGTGAAGGTGAGGGAAGCCCCTCACCTTGGGTCTTTTGCTGTGCCTCCTATGTATGTCTGGTT
  TGCTGGAAGAGTGATTAATACATCTTTAATTTATTAAAAAACAATGTAGACCTTTAAACTTCAGTCTTA
  TTGGTAATAAAAGGGAACTTAATTCATACAGGTACTTGATACAGTTATACATTTTCCACTTACAAAAAG
  AAGACAATTCTGTTAAATGAAACGTGTATCGTAAAATGTATTTTTATTTTACCCACGAGAATGTTGTTA
  TTTTTAGCAAATAGAACTCAATGCAGTGCATTGGTTATTACCCTGTGTACCTTGTCCCTCATTTTGCTG
  TGACACCCTGAAAAAGCTGACCACAAATGCAGTATTATCTTGACATACCTCTGTCCTCCTCAGTGCTTT
  TTAATGAAATTTCACCTGCCCCTTGTGTTCCTGCTTTGGATAGTTTTTGCTCTTAAGCATTGGCATTCT
  TCTATTGTATGGTATGTATTTATAGCAATTGTAGGATGGTCTGAAATGTCCACACACTTTCTATGCATA
  CAAATTTTGTGAATTCCCAAAGTATGCTTTGGAATTGGTCATAGTCTTTTCGTATCATGAGTGTGTATC
  CCAGAAGATACAGAATGCGGTCATTTTACCTGAAATTATTTGAGAACACTGGATGTATCTGGTCTGTGT
  AAAAAGTTAGCACTTACCCTCTTTTCTTCCTGGCCAAGCCCTCTGAATTCATCTGTTAATATCAAATTG
  TATTTTTGCCACATTTCTGTATTGGTGAAAAGCGAATCTGCAGAATAACTGTCAACGTTTTTCATTCTT
  GTTTTACAAACCTATTTTTTAAAGTGAGAATAAGGTTGGTTTTTACCAAATATTTGTTCCTCCTTGACA
  AAAGTAGCTCTGTGTGGATAATATGATTTTATTACTACAGTTCCAGTCACTTGGTTATATTTATCTTAG
  CATGAGCCTTTCACACCAAGATTTCTATATTTTGTAACATAGGTGAAATATTTAAAACATCAAAAACTG
  TAAATCTAGATTTTTAAAAAATCCAACTGCAATGTCTTTTCCTTTGATTGAGATGATTTGTGTAAACTC
  ACCAGTCTTGCTTTGGAGTGAGCAGAAGAGAATGACTATTTTACGTGGAGCATCATTGTGTGACTGTTG
  ACCTGGACAGTCCCAAGGGCTATGCAGATGGACTCCATTGGCACAGGGAGGTTTGACCTCTTCCCTGCT
  ATTATCCCTCCTCCCAACTGTACAGATTTGTTTGTTGTAGTTTATGTACTTCTTGATACTGTCAAAAAA
  TTATCTGGAAATGGTTTTATTTTGTGAAGTGAACAATACTTTGTAATTTATGATAGTGTAAATGGAAGG
  CAAAGCATCACATGTATTAAATTCTTCTGTCTATTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001039936.3
• Summary: This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that contains a phorbol-ester/diacylglycerol (DAG)-type zinc finger, a Rho-GAP domain, and an SH2 domain. The encoded protein translocates from the cytosol to the Golgi apparatus membrane upon binding by diacylglycerol (DAG). Activity of this protein is important in cell proliferation and migration, and expression changes in this gene have been detected in cancers. A mutation in this gene has also been associated with schizophrenia in men. Alternative transcript splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, May 2014]
• Locus ID: 1124
• MW: 61.9