DAZAP2 (NM_001136267) Human 3' UTR Clone

CAT#: SC214880

3' UTR clone of DAZ associated protein 2 (DAZAP2) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DAZAP2
• Synonyms: PRTB
• ACCN: NM_001136267
• Insert Size: 1505 bp
• Sequence Data:
  Insert Sequence

  >SC214880 3’UTR clone of NM_001136267
  The sequence shown below is from the reference sequence of NM_001136267. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GGTTCAGATGGTGGCTACACCATCTGGTGAGGAACCAAGGCCACCTCTGTGCCGGGAAAGACATCACAT
  ACCTTCAGCACTTCTCACAATGTAACTGCTTTAGTCATATTAACCTGAAGTTGCAGTTTAGACACATGT
  TGTTGGGGTGTCTTTCTGGTGCCCAAACTTTCAGGCACTTTTCAAATTTAATAAGGAACCATGTAATGG
  TAGCAGTACCTCCCTAAAGCATTTTGAGGTAGGGGAGGTATCCATTCATAAAATGAATGTGGGTGAAGC
  CGCCCTAAGGATTTTCCTTTAATTTCTCTGGAGTAATACTGTACCATACTGGTCTTTGCTTTTAGTAAT
  AAAACATCAAATTAGGTTTGGAGGGAACTTTGATCTTCCTAAGAATTAAAGTTGCCAAATTATTCTGAT
  TGGTCTTTAATCTCCTTTAAGTCTTTGATATATATTACTTGTTATAAATGGAACGCATTAGTTGTCTGC
  CTTTTCCTTTCCATCCCTTGCCCCACCCATCCCATCTCCAACCCTAGTCTTCCATTTCCTCCCGCCAGT
  CTCCATTGAATCAATGGTGCAGGACAGAAAGCCAGTCAGACTAATTTCCTTCTTTCCTCGCACTTCTCC
  CCACTCGTCATCTTTTAACTAGTGTTTCACAAGGATCCTCTGAAACCCTCTCTGTGCCCCAAGTACAGA
  TGCCATTACTTCTGCTTTCGTATCTCCTCAGGCAAAAGTGGAGGGTGCCTTATGGGCCCTCCTCATAGG
  TTGTCTCTGCATACACGAACCTAACCCAAATTTGCTTTGGTGCCAGAAAAACTGAGCTATGTTTGAACA
  AAGATGTCGTGCAAACTGTACTGTGAACAACAGTTGGTTTAAAATATGAGGGGCAAGGAGGAGGATGCA
  TTTCAAAAGCTTGATTGATGTGTTCAGAGCTAAATTAAGAGGAGTTTTCAGATCAAAAACTGGTTACCA
  TTTTTTGTCAGAGTGTCTGATGCGGCCACTCATTCGGCTCCCCAGAATTCCTAGACTGGGTTAATAGGG
  TCATATTGTGAATGTCTCACTACAAAATGACTTGAGTCCAGTGAAATCTCATTAGGGTTTAAGAATATT
  TCAGGGATCCTTAATGTTTTGATTTTTGTTTTCTGAAATTGGATTTTATTTTATTTTATCTTATAATTT
  CAGTTCATCTAAATTGTGTGTTCTGTACATGTGATGTTTGACTGTACCATTGACTGTTATGGAAGTTCA
  GCGTTGTATGTCTCTCTCTACACTGTGGTGCACTTAACTTGTGGAATTTTTATACTAAAAATGTAGAAT
  AAAGACTATTTTGAAGATTTGAATAAAGTGATGAAGTTGCATTACACCTCACTGCAAGGATTCTTTACT
  TAGCTTGTTTTTAGATTTCTTCTATATATATTTTATTTATATCCCATCTAGAATTCAGCTAGGTGCTGC
  TGCTGCTCTGTTTCCTTTGATGACGCTTTGAAATAAAGGCAGGAGTACAAGCCTAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001136267.2
• Summary: This gene encodes a proline-rich protein which interacts with the deleted in azoospermia (DAZ) and the deleted in azoospermia-like gene through the DAZ-like repeats. This protein also interacts with the transforming growth factor-beta signaling molecule SARA (Smad anchor for receptor activation), eukaryotic initiation factor 4G, and an E3 ubiquitinase that regulates its stability in splicing factor containing nuclear speckles. The encoded protein may function in various biological and pathological processes including spermatogenesis, cell signaling and transcription regulation, formation of stress granules during translation arrest, RNA splicing, and pathogenesis of multiple myeloma. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
• Locus ID: 9802
• MW: 56.8