Ryanodine receptor 2 (RYR2) (NM_001035) Human 3' UTR Clone

CAT#: SC214041

3' UTR clone of ryanodine receptor 2 (cardiac) (RYR2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RYR2
• Synonyms: ARVC2; ARVD2; RyR; RYR-2; VACRDS; VTSIP
• ACCN: NM_001035
• Insert Size: 1371 bp
• Sequence Data:
  Insert Sequence

  >SC214041 3’UTR clone of NM_001035
  The sequence shown below is from the reference sequence of NM_001035. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CGGAAACAGTATGAAGACCAGCTAAATTAAACTCAGACCCAATCACCTCTAAAAACCAAAACCCTACCC
  CTCTCTCTCCCTCTCTCAATTTCTCTGCTCTCTTGGAAACATTTTGCTGATTTTGTGAATTGCCAGCGT
  TGTGTGTTTTCTGGGAGCATCGAAGCTCTGTTTCGGAAGAGCTGTTTCCTCCCCCCACCTTTTGTATTT
  ACTTTGAGACTAAAGACTGAAGAATAATCTAAATTCATACTCAGACAAAAAAAGGAATTCTGGAAAGAA
  AACCATTCTGGACACTGTCATAACACACATAGATAGATTTTCTTCTGAGACTCCCGGAGTCTTCTCGAG
  CTACGAGACCTTCACAGAGACACGTGGCAGCCACACTCACCCAGCCTCTTTATTTCACCATCCTGGAAG
  GAAACTGTCTGTCTAATGGTCACAGAGCACTGTAGCACTTAACAGATTGCCATGGACACCAGTTGCGAA
  GGGAAATAGTGCCTTACTATATGTGGGTTGAGCTATGCAGAAGATACGTGCATGAAAAAACATCTTTAT
  TTTCTTTATGTCGACCTTTCTTTTCTTAGATTGATTTTGTGAGGTTTTTTTTTTTTCCTTTAGTCTTTT
  CTTTAGTGGGGGAGGGTAAGAAAAGCAGTTTGCACTTAAAAAGAAAAAAAAAAAACGGGTGGTGTGTCT
  CAGGACAAAAGGAGGCTCTTCTCATTCAGCTAAATTCACATTTGCCCCCCCCCCCCGCCCCCGCCCCCA
  TATGCTCCTGCTATTATTTTGGTAATGCTCTGATGCAACATTGCAACTTTATGAAATCTTTGTATGAAA
  ACAAAAAGACTGCAAAAAATACACTTTCAAAAGAAACCATTCATTGCATGTTTATTATGCAAGTTTAAA
  TGAACAAAGAAAACCTTCAGAAACAAGTTGATCAACGTGAGAGAAATTTCATGATAATTATTCATAGTA
  ATAAAGTGTCGTGGGCTTAATTGTATATCTGGAGCCAGTGTCATCCACCAACAATGTGATACAATATGA
  ACTTGACAGTAGTTTTGGGTTTTGCTCATTCTTTCAGCCACCTGTGATCAGTTTTGTTGATTAAGGACT
  TCTTGTCAGGCCATTTTTTAATATCAAAGCTGAAGCAATATCCATTCAGGGATTTCATCAGTTGCATCA
  CAAAACACGGATGATAATATACATCACTCCATTTTTTCCATTTTGAGTCCTTTTAAATGTAATGCTAAC
  CTTTACAATTAAAAAGTCAGATTCAGAATGGAAACAAGGTCATTTTGCAAACATTGGAGCTCTTTTATT
  ACAAGTCTGCTTGTTAATTTTAGAACTGTAAAACCGCTCTGATTAAACTATTTAACTAAC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001035.3
• Summary: This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia. [provided by RefSeq, Jul 2008]
• Locus ID: 6262
• MW: 53