Choline kinase alpha (CHKA) (NM_001277) Human 3' UTR Clone

CAT#: SC212759

3' UTR clone of choline kinase alpha (CHKA) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: Choline kinase alpha
• Synonyms: CHK; CK; CKI; EK
• ACCN: NM_001277
• Insert Size: 1159 bp
• Sequence Data:
  Insert Sequence

  >SC212759 3’UTR clone of NM_001277
  The sequence shown below is from the reference sequence of NM_001277. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TTCCACCAGAAGAGGAAGCTTGGGGTGTGACTGTGGGGAGGACTCCATCCACCTCATCACTGGACTGCA
  TGGGGAGGCAGCAGAGCGGGGTCCCCTCTGTGCTTCGACTACTGCTCCTGTGGCAGGAGGCTTTGGGTG
  GCTCACTACTGAACACATGTGTATGATACTAAAGACGGTATTAAAATGGAGCGACGTTTATTTCATCTC
  TTGTTTACGATTTCACTAGGACTCAGAAACGAGATCGGGAAGCAGAAATATAGTGCAATAGTGCAACAT
  CTCTGAATCCTTTTAATCTAGAGAAGGCATTTCATATTTGGGGGCTAAGGTTTCCAGTCAGATGAGGCA
  AACAGCAAGAGTAAGCAGTGTTACTTGCAGGTACTTTGGTTAATGTTGATTTAAATTTTCATGAATGTG
  CTGGTGAACACTGTGACCAGGCTTTTGTAGATGGCGATGTGTTATAGACGGTGCTCACTCCCAAGGGAC
  AGCAAGTGAGCAGAGATGTACTGCAAAGTCGCCAGTCACTGCTGCAAGGTGGCCTCTGCCTGGGGCCTC
  CAGAAGCTGCTCCTTTACCCTCTTGGTCCCATGGCTGAAGCTGGAGCAGCGGATTGCTCTGGAGCAGCC
  AAGGCCGCCAGCGTGTGGAGCAGAGCTCTCCCCTCCTGCTGGGCGTGTGTGACACTGATGAGTTTCACT
  GTACTGCATGTGACTTCTCCCCTGCCCTTCCTCCTGATGGAGTGTGCAGACAGCCATGCGTGGCCACGG
  GGGCAGTGTGAGGACCTCCCTGTCTCCCGGCTCCCCTCCCAGGGGAGCCAGCTGCTTGACCTAGCTCTT
  TGGGCCTCTCCTGCCCTCTGCTCTGCCTGGAGTGTCGGATCCTGTGAGTAGGCTGGGCCTCCCCTGGGC
  AGGGTTCTCCAAGGGCCCGGTTTCCCGGCCCTTACCTTTCCTGATGCCCCTGACATCATCATTCTTGTG
  GGAGACAGCAGCCTGTATGTGGTGTGGGGCGTGGATCGAGTGTAGCTGTGAAATCCATATATATGAAAT
  GTCCTGCGGGATACAGTCTTAGCTGACTTTTTTTTACTCTGAACTCTTATTTGAATTGTTTTTTGTGCA
  TATATTTCTGCTACCACAGAGATTGTACTATACAAATAAAAAAATAAAAACCCAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001277.3
• Summary: The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 1119
• MW: 43