Brk (PTK6) (NM_005975) Human 3' UTR Clone

CAT#: SC212598

3' UTR clone of PTK6 protein tyrosine kinase 6 (PTK6) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PTK6
• Synonyms: BRK
• ACCN: NM_005975
• Insert Size: 1565 bp
• Sequence Data:
  Insert Sequence

  >SC212598 3’UTR clone of NM_005975
  The sequence shown below is from the reference sequence of NM_005975. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AGCTTCACCAGCTACGAGAACCCGACCTGAGCTGCTGTGGAGCGGGCATGGCCGGGCCCTGCTGAGGAG
  GGGCCTGGGCAGAGGGCCTGGACCTGGGATCAAGGCCCACGCGCTTCCCTGGGGTTTACTGAGGTGATG
  GGTGCAGGAAAGGTTCACAAATGTGGAGTGTCTGCGTCCAATACACGCGTGTGCTCCTCTCCTTACTCC
  ATCGTGTGTGCCTTGGGTCTCAGCTGCTGACACGCAGCCTGCTCTGGAGCCTGCAGATGAGATCCGGGA
  GACTGACACGAAGCCAGCAGAGGTCAGAGGGGACTCTGACCACAGCCCGCTCTCTGGCTGTCTGTCTGC
  AGTGCCCGGCTGAGGGTGGGAGGCAAACACGCCTTGTTCCTGCTCTTCCCAGTTCAGCTTGGTGGGAGA
  AAGTCATTCGCGTGGCTCGGGACGCTCATGTAAATTTGGTTTTGGTGCTCAAGGGTTCTTTCCTCCCAG
  GGGCAGGTGTTTCTTTCCTGTTTGTCTTGTGTCTTGAGAGCTTGGCCTTATGACCAGTGAGAACTCTCT
  CCCTGGTCTCTGCCAGCCCAAGCATCACTGCCCGAGGCGCCAGCTCAGTTTCACCGTCCACGTCCACAA
  GGGGCTTTTCCCACCTTCACCTTTGTCGCTGGGTCAGTGCTGGAAAGCGCCCCTCACTCCTGCGCTGAC
  AAGGGCCCTTCTCTACTGTCTGTGGGGTGGTTCCGGGCTGGGGGGGCTGCCTCCTTTGCACCTGATTTT
  GAAGGTGTCTCTTTCATCCATGGTTAAGTCATAAAAAGCTTATTGGTTTTGGTTTTGACTCACCTGAAA
  GTTTTTTTGGTTTAAAAGAAGAATAGGCGGGGCACGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGA
  GGCTGAGGCAGGTGGATCACGAGGTCAGGAGATCGACACCATCCTGGCTAACACGGTGAAACCCCGTCT
  CTACTAAAAAATACAAAAAATTAGCTGGGTGTGGTGGTGGGGGTGGGCGCCTGTAGTCCCAGCTACGTG
  GGAGGCTGAGGCAGCAGACTGGTGTGAACCCGGGAGGTGGAGCTTGCAGTGAGCCGAGATCGCGCCACT
  GCACTCCAGCCTGGGCGACAGAGCGAGACTCCATCTCAAAAAAAAAAAAAAAAAAAAGAAAGAAAGAAA
  AATAATTTTGGGAGTTTCTGGAAAGGTACTAGGATTTCTCAAAAGGATTTGTCTTCTGCCTTGTGAAAG
  ACAGATGTCAGACTAATCAGGCTTATCCGATGTGCTACATGAGATGGAAAGCGTGTGAAATAGTAAGTC
  ACACTAAGTCTTCTGGAGGTTCTATTTACGGGTTTGGTTTTGATATGAATGTTGCAGAAACTCTATAAA
  ATTCCTAGAAATCTGATACGTTATCCTATGATATATATCCTATCTCACATGCTATTCCTAGAAATCTGG
  TGCAATGTTATCAGTCATAATTTTGGTTATTACATTAAAATGCTGCATGCCACAGAAATAACCAAGTTT
  CCTTGCCAACCGTGTCACGAATATAATAAACTCTCATCAGATCCTGA
  AGCGGACCGACTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCC
  CAACCTGCCATCACGAGATTTCGATTCCACCGCCGC
  
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005975.4
• Summary: The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
• Locus ID: 5753
• MW: 57.3