PACE4 (PCSK6) (NM_138323) Human 3' UTR Clone

CAT#: SC211871

3' UTR clone of proprotein convertase subtilisin/kexin type 6 (PCSK6) transcript variant 5 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PCSK6
• Synonyms: PACE4; SPC4
• ACCN: NM_138323
• Insert Size: 1052 bp
• Sequence Data:
  Insert Sequence

  >SC211871 3’UTR clone of NM_138323
  The sequence shown below is from the reference sequence of NM_138323. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AACCCGGAGAAGCAAGGGAACCTGGACTAAAACACGTGTTCCGGTGGCAGATTGAACAAGAGCTTTGGT
  AATCTCTGTGAAATACTGCAGAGGGACAGATGACCAAAGCCACCACATTTAGAACTTTGGCTGCCTTTG
  GAAGTCCAGAGCTGGATCTCTCAGCTCCCGCCCCCAGAGGCTCAGCACTTTGGACATGGCTCACAAACA
  GTTGTTGATTGACTGCATGAATGCGTGTGCGTGCAAGCATGAACCTTGTTTAAATCAAGAGCTTACATA
  ATTTTAACCAGTTCTGTCTTCAGCTGTACATACTCAGTAAAATGTTTAATGAAGGGGAAGAGATTAGTC
  TCTTCTGTGTGACCATGTTTTCCCTTTATTCATCCTAAAAAGTTCCATGAATTCTTGATTTCCTTTCAG
  TGGCCCTTTCAACAATGTCTTTTTTCCCAAGAGCATAACTGTTCTCATTTTATTGCTAGCCATCTTGAT
  CTGTGTTTTATTGACATCTCTTTTGAGCTAATCTTCATTTCTAAGATAAGAGTTGAGATTTTGCAATCT
  GTGTTCGATGGCTCAATCTATCCTGTGCTTGATGCTAGAAAGGAAGACAGATTTAAAGCACATGCCTTC
  TGTGCCGGCTTTCAAGTTTGTCACTAAACTCTCATTTCTGGAAAGTGCAATTATAGAGTATCACTCCCA
  CTTCCTTGGAAACAGAGCTGAAGAACTTGGCACACTCTCCAAACAGTCACCATACACACTGTTGTCAAA
  AAGTTCCATTTTTAACCCCATTTGCATTAATATTGCAGTCAATCTCTTTACCTCCTTTTCTCTTTCACG
  GGGCCGTGACAGTGACGCCTTTCCCCAAAACTCTCCTCGTTTGAGAAAAAAAGAAGTATGTATCCCCAC
  TTATCTCGGGGAGAAATGCAACCAACTGCTGCTGTGCACATTTATGAATCACAGTATTGTTTAGTCGGT
  TCTGTATCTCCAGTAGAAAGCATAACAAAAAGATGACCTTTGTCTCACCTCATAGCTAATTTTTGCAAA
  TAAAATACTAAACATTG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_138323.3
• Summary: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. The encoded protease is constitutively secreted into the extracellular matrix and expressed in many tissues, including neuroendocrine, liver, gut, and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include transforming growth factor beta related proteins, proalbumin, and von Willebrand factor. This gene is thought to play a role in tumor progression and left-right patterning. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2014]
• Locus ID: 5046
• MW: 40.3