DSN1 (NM_001145316) Human 3' UTR Clone

CAT#: SC211682

3' UTR clone of DSN1 MIND kinetochore complex component homolog (S. cerevisiae) (DSN1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DSN1
• Synonyms: C20orf172; dJ469A13.2; hKNL-3; KNL3; MIS13
• ACCN: NM_001145316
• Insert Size: 1023 bp
• Sequence Data:
  Insert Sequence

  >SC211682 3’UTR clone of NM_001145316
  The sequence shown below is from the reference sequence of NM_001145316. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CATGGATCTGGATCTGGATCTTGTCAGTGACTTTATGAGAGTTTCTGCCACAAGGTGCCCAAGAGGAGA
  GGAATGGGAAGAGTGCCCCAGCACGTGGTGACTGCGTGATTTCTGCTCGTTGCCTTTGAAGATAACTGG
  CAGGACTGACTGTAGAACACTTTGACTTTTTTCAAAAAGTGATGGAATTTGTACATCCAAATGAATATT
  GTATAGACAATTTTCCCAGGAATGTGCAAAATGCTTGAAAGTTCAAACTTCTTTTTTGAAATGATCTTC
  AGATCCAGTGGCCCATTCTTTTATCTTTATCCTGTGAAGGTGTTTTTCAGGTTTTGAAACAATCCAAAA
  ATCATTTAGGACCAAGTCTAAGGAAACATTTTAGTGGCCAAGTTGGATTCCGATTGTAAAGGAATGATA
  CTAATTTTCTAGCATGGCTCTGAAGGTGATTTTAGGTAGAAGAGTTTTGAGGCTGGGCGCAATGGCTCA
  CGCCTGTAATCCTAGCATTTTGGGTGACTGAGGCGGGTGGATTGCTTGAGCCCAGAAGTTGAAGACCAG
  CCTGAGAAATAAGGTGAAACCCTGTCTACAAAAAATACAAAAAGTTAGCTGGGTGTGGTGGCGTGTGCC
  TGTAGTGCTAGCTACTCAGAAGGCTGAGGTGGGAGGATTGTTTGAGCCCAGGAGGTTGAGGCTGCAGTG
  AGTTCTAATTGCGCCACTGCACTCCAGCCTGAGCGACAGAGTGAGACACTGTCTTAAAAAAAATTAAAA
  ATTGTAAAAAAATGAAAAAAAAAGTTTTGAGCATTATTTGCATCATTGGGATACATATGTCACTTCACA
  AGATGTTCAATTTGAAGGAAATACCACTCATTCTCTATGTCCTGTTGTCTGTAGTGTGCTTCAGTTTTT
  CATATTGAGTTGACCTAAATCCTGGATTCATGACAAGAAAGGAGTAAGTACTACTATTCATTGTTCTAT
  TTGTTTATAATCTGTATTATAAAATTGCACATAATTAAAAGCTTTCCCTTGTCTTCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001145316.2
• Summary: This gene encodes a kinetochore protein that functions as part of the minichromosome instability-12 centromere complex. The encoded protein is required for proper kinetochore assembly and progression through the cell cycle. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009]
• Locus ID: 79980
• MW: 38.8