Phospholipase C beta 2 (PLCB2) (NM_004573) Human 3' UTR Clone

CAT#: SC210306

3' UTR clone of phospholipase C beta 2 (PLCB2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PLCB2
• Synonyms: PLC-beta-2
• ACCN: NM_004573
• Insert Size: 836 bp
• Sequence Data:
  Insert Sequence

  >SC210306 3’UTR clone of NM_004573
  The sequence shown below is from the reference sequence of NM_004573. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAGGCAGATGCCCAGGAGAGCCGCCTCTGATGCCCCCATCCCACTGGGACATTTAGCAAGGAGGTTCAG
  CCCCTTCTCTGGGATGTGGTTCTATTCCCCCAGGAAAAAGGAGCCCCAGCCTTCTGAGGCTGTGGGAAC
  CTGTGGCTGCCTTGGACGCTGCAGCCCCCTCCTCAACGGCCAGGCCAGAGTCTGAGACAGGACCCAGGC
  ACCCTCACGGCAGGGCCTCTCTGGGGCCTAGAAGTCTTCTCAAGCTGACTTCCTACCTCCCCCTCCATC
  TCTAGATAAGTGTCATATATTTGTTGAGGGCAAAAGACTATGGACTGGAAGGCAGAAAGTGGGATCCTG
  GCCCCACTCTGCCTTTCCTATTGAGCAACCAGCTCTGGGCTCAGTTTCCTCACCTGGAGGGTTGGACCC
  ACTCACCTCCACTCTAGCCCCGAACCCTCCTGCCCCAGGCTTCCAGGCCCCATCAGGCCTGCCCTGAGT
  TGGCCTTGTCCACTCCTTGAGGCAGATCCTGGCACTACCTCACAGCTCCCTGGGGACGGCCACTCCCTG
  GCTGAGGGCCCTCCCCTCCCCTCTGCCTGTCCGGAACGGGAGGCTGAAATGGAAAAGCTGCCTTGGCCC
  TGCTTGGCTGAGTCACAAGGGGCAGTGGGCTCTTGGGTGCTGTTCCACCCTGACCCTGGCTCACCCCTC
  TTCCTAGGCCTGGGGGCAGGCAGTTCCTACCATGTACCCCTCTCAGGCTGCCTGCCTGACAAGGTCAGC
  ATCATTTGCTCTCCTGAATTTATGAGGTTTATTTATTTTTCTCTTTCCTACTCCTATTAAAGAACCTCG
  TCCCAGTG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004573.3
• Summary: The protein encoded by this gene is a phosphodiesterase that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate to the second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol. The encoded protein is activated by G proteins and has been shown to be involved in the type 2 taste receptor signal transduction pathway. In addition, nuclear factor kappa B can regulate the transcription of this gene, whose protein product is also an important regulator of platelet responses. [provided by RefSeq, Jan 2017]
• Locus ID: 5330
• MW: 30