B Raf (BRAF) (NM_004333) Human 3' UTR Clone

CAT#: SC207797

3' UTR clone of v-raf murine sarcoma viral oncogene homolog B1 (BRAF) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: BRAF
• Synonyms: B-raf; B-RAF1; BRAF1; NS7; RAFB1
• ACCN: NM_004333
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC207797 3’UTR clone of NM_004333
  The sequence shown below is from the reference sequence of NM_004333. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GGGGGATATGGTGCGTTTCCTGTCCACTGAAACAAATGAGTGAGAGAGTTCAGGAGAGTAGCAACAAAA
  GGAAAATAAATGAACATATGTTTGCTTATATGTTAAATTGAATAAAATACTCTCTTTTTTTTTAAGGTG
  AACCAAAGAACACTTGTGTGGTTAAAGACTAGATATAATTTTTCCCCAAACTAAAATTTATACTTAACA
  TTGGATTTTTAACATCCAAGGGTTAAAATACATAGACATTGCTAAAAATTGGCAGAGCCTCTTCTAGAG
  GCTTTACTTTCTGTTCCGGGTTTGTATCATTCACTTGGTTATTTTAAGTAGTAAACTTCAGTTTCTCAT
  GCAACTTTTGTTGCCAGCTATCACATGTCCACTAGGGACTCCAGAAGAAGACCCTACCTATGCCTGTGT
  TTGCAGGTGAGAAGTTGGCAGTCGGTTAGCCTGGGTTAGATAAGGCAAACTGAACAGATCTAATTTAGG
  AAGTCAGTAGAATTTAATAATTCTATTATTATTCTTAATAATTTTTCTATAACTATTTCTTTTTATAAC
  AATTTGGAAAATGTGGATGTCTTTTATTTCCTTGAAGCAATAAACTAAGTTTCTTTTTATAAATTTTGA
  GTGCAGGTGACCAAAAATATTGCTGAGGAGTGGCACGTTTGACATGAGTAAAATGTCTTAACTTCGGAT
  TTTTAGCGGGAAAATGTTATAAATTGGAGTTTCTTTTAAATAGCTTTTTTTAAAATACATTAAGGATGT
  CTCGCTCATGTAGAAGTCAAATTTTGTTGCAAACGCATTGCTCCCTTCACACCCAATCTCTCCCCTGCA
  AAAAATCTTCACAGAATTCTGTGAGAACTTTTAGGTGTGTTTTTCTTTGAGATACCTCTGGTTGCCAAA
  CACCAGGTAATAGATTTTTTAAAGTTGTTATTAGATTATTCTTACCTCTCATGATGCATATTTTAGCAA
  TCACCTTATCATTGTGTCTCATGTTCTGTCCTCCTTATATTCTTTGCCCAGCAAGATTCTACTTATGAT
  GAATGAATGCTCTTCTCCTTTTTTCATTCAATGGTATGAAGTATTTGTTAGGGTTCTTTAGTACTTACA
  CTTTGTTGTGTAGAAAATGACTGTAATGTGGTGGTCAGTGTATTCTTACTGTGATTCAGAGGGAATCAA
  AAGTAGAAAGCAACAGCACGTGGTCCTATCAAAGATTTGGCCATCTCTGCTTCACTGTCAGCCTCTTAA
  CTATATCTTCACTTACTCAATTTGGTTTTGTCATGATTTTTAAATGTAGCCAATAGATCAAGGTTCTTC
  CAGTAAACACATATCTGCATAAATGCCTCCTTGAAGTCAATAAAGAAGGAAATTGAGAAGACTTTAAAT
  TAATGATAATTTAGTTTTTAAGTACCCACAAATAAATTTTTGAAACATTTTCTTTATTTGAATACTTAG
  ATGTCATCCAGGAAAATCACTCAATAATAATTACGGCAAATCTTTAACCCCTCATTTGGGTAGCTTAAG
  ATAAGTAATGCCATTATGAATCAGAATTGATTCATGACTTTAGTTAAGAAAATGAAAAGGAACATTTCA
  CGTATTTTTAAAAATGATACTAAGGAATAAAGAAGTACAACTATTGGAAAATATCTAAGTATATGATTT
  TTAAATCCTCCAGTGGCATTAAATATATGATTATTAGTAATTGTTAGATAGGGTTTTATTCATTCACAA
  ATAGAAGACTAGCAAGCATGTAACTAACAAAGTTTTTACAAAATTGACTTTGTGGAATGCTCCAAATGT
  TTGGCCATTTTGAGGCACAAGGTCAGGGGTCTCTTTATTGATAGAGCTCCTTCTATAATTTCCCAGCAT
  ACCTGCCTCACAGTTATCTTCCTTTCATTGTTCACTCTCTTTTTCTTCTCAATGCCATCCTGCCTAGGC
  TCCCATCATCTGCATCTGACACCTTTCCTTTCTTTCTTTACTAGTCTCCTTTGCGATGGGTGTGGCTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004333.6
• Summary: This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]
• Locus ID: 673
• MW: 77