Human IgA (Secretory component, free and bound) Sheep Polyclonal Antibody

CAT#: TA355211

Human IgA (Secretory component, free and bound) sheep polyclonal antibody, HRP

Specifications

Product Data

• Applications: E, ICC, IHC, WB
• Recommended Dilution: This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
• Reactivities: Human
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified free human secretory component isolated from pooled milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in human secretions. In immunoelectrophoresis against human milk, using a high electroendosmosis agar plate, free SC is precipitated in the alpha-2 region. The immunoconjugate does not react with other molecular forms of IgA, or with any other secretory or plasma protein. In immunocytochemical and immunohistochemical staining for the detection of free SC and secretory IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA) to identify and measure SC or secretory IgA in human body fluids.
• Formulation: Horseradish peroxidase-coupled purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
• Reconstitution Method: The antibody is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
• Conjugation: HRP
• Storage: The lyophilized conjugate can be stored at +4°C short term; prolonged storage at or below -20°C.