WIBG (PYM1) Mouse Monoclonal Antibody [Clone ID: UMAB208]

CAT#: UM800100

WIBG mouse monoclonal antibody,clone UMAB208

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-WIBG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, UM800100) (1:500)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WIBG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, UM800100) (1:500)

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  Immunohistochemical staining of paraffin-embedded ovarian cancer with mouse anti-WIBG clone UMAB208 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic.

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  Immunohistochemical staining of paraffin-embedded liver cancer with mouse anti-WIBG clone UMAB208 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic.

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  Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500

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  Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-WIBG monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen). (1:500) Dilution: 1:500

• Immunofluorescent staining of HeLa cells using anti-WIBG mouse monoclonal antibody (UM800100, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-WIBG mouse monoclonal antibody (UM800100). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB208
• Applications: 10k-ChIP, IF, IHC, WB
• Recommended Dilution: IHC 1:100~500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human WIBG (NP_115721) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.5 kDa
• Gene Name: PYM homolog 1, exon junction complex associated factor
• Database Link:
  NP_115721
  Entrez Gene 84305 Human
  UniProt ID Q9BRP8
• Background: WIBG (within bgcn homolog (Drosophila)) is a protein-coding gene. Diseases associated with WIBG include mucoepidermoid carcinoma, and tongue cancer. GO annotations related to this gene include ribosome binding and RNA binding.
• Synonyms: PYM; WIBG