VILIP1 (VSNL1) Mouse Monoclonal Antibody [Clone ID: UMAB115]

CAT#: UM800034

VSNL1 mouse monoclonal antibody,clone UMAB115

Product Images

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (UM800034; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded human pancreas using anti-VSNL1 clone UMAB115 mouse monoclonal antibody (UM800034) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using

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  Immunohistochemical staining of paraffin-embedded human glioma using anti-VSNL1 clone UMAB115 mouse monoclonal antibody (UM800034) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using p

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  Immunohistochemical staining of paraffin-embedded human skin using anti-VSNL1 clone UMAB115 mouse monoclonal antibody (UM800034) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pre

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  Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Colon, 9: Spleen, 10: Thyroid). Diluation: 1:500.

• Immunofluorescent staining of HepG2 cells using anti-VSNL1 mouse monoclonal antibody (UM800034, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

• Confocal immunofluoresce image of primary rat neurons labeled with anti-VSNL1 mouse monoclonal antibody (UM800034, green, 1:100). Actin filaments were labeled with TRICT-Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

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Specifications

Product Data

• Clone Name: UMAB115
• Applications: IF, IHC, WB
• Recommended Dilution: IHC 1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-191 of human VSNL1 (NP_003376) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22 kDa
• Gene Name: visinin like 1
• Database Link:
  NP_003376
  Entrez Gene 24877 RatEntrez Gene 26950 MouseEntrez Gene 7447 Human
  UniProt ID P62760
• Background: This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
• Synonyms: HLP3; HPCAL3; HUVISL1; VILIP; VILIP-1

Reference Data

• Protein Families: Druggable Genome