HSP27 (HSPB1) Rabbit Polyclonal Antibody

CAT#: TA890054

Rabbit polyclonal anti-HSPB1 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPB1 (RC201800, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPB1 r. Positive lysates LY400587 (100ug) and LC400587 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from different cell lines or tissues by using anti-HSPB1 rabbit polyclonal antibody (TA890054).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and HSPB1-Knockout Hela cells (KO, Cat# LC810109) were separated by SDS-PAGE and immunoblotted with anti-HSPB1 monoclonal antibody TA890054, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human HSPB1
• Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: 2.43mg/ml
• Purification: Purified from the immunized serum by affinity chromatography (Protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.6 kDa
• Gene Name: heat shock protein family B (small) member 1
• Database Link:
  NP_001531
  Entrez Gene 15507 MouseEntrez Gene 3315 Human
  UniProt ID P04792
• Background: The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
• Synonyms: CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; SRP27

Reference Data

• Protein Pathways: MAPK signaling pathway, VEGF signaling pathway