GLCNE (GNE) Rabbit Polyclonal Antibody

CAT#: TA890043

Rabbit polyclonal anti-GNE Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GNE (Cat# RC222626, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GNE antibody(Cat# TA890043). Positive lysates LY417278 (100ug) and LC417278 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from different cell lines or tissues by using anti-GNE rabbit polyclonal antibody (TA890043).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human GNE
• Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: 4.29mg/ml
• Purification: Purified from the immunized serum by affinity chromatography (Protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 79.1 kDa
• Gene Name: glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
• Database Link:
  NP_005467
  Entrez Gene 50798 MouseEntrez Gene 114711 RatEntrez Gene 10020 Human
  UniProt ID Q9Y223
• Background: The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
• Synonyms: DMRV; GLCNE; IBM2; NM; Uae1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Metabolic pathways