YAP1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI5E6]

CAT#: TA813309AM

YAP1 mouse monoclonal antibody,clone OTI5E6, Biotinylated

Product Images

Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and YAP1-Knockout HeLa cells (KO, Cat# LC832485) were separated by SDS-PAGE and immunoblotted with anti-YAP1 monoclonal antibody TA813309 (1:1000). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Western blot analysis of extracts (35ug) from 5 cell lines lysates by using anti-YAP1 monoclonal antibody (1:1000).

Specifications

Product Data

• Clone Name: OTI5E6
• Applications: WB
• Recommended Dilution: WB 1:1000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 347-504 of human YAP1 (NP_001123617) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.3 kDa
• Database Link:
  NP_001123617
  Entrez Gene 22601 Mouse
• Background: This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]
• Synonyms: COB1; YAP; YAP2; YAP65; YKI

Reference Data

• Protein Families: Druggable Genome