RAD21 Mouse Monoclonal Antibody [Clone ID: OTI6C10]

CAT#: TA811222

RAD21 mouse monoclonal antibody,clone OTI6C10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RAD21 (Cat# RC208262, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD21 (Cat# TA811222)(1:2000). Positive lysates LY401887 (100ug) and LC401887 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RAD21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811222) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-RAD21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811222) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RAD21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811222) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RAD21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811222) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RAD21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811222) (1:2000)

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Specifications

Product Data

• Clone Name: OTI6C10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 193-458 of human RAD21 (NP_006256) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 71.5 kDa
• Gene Name: RAD21 cohesin complex component
• Database Link:
  NP_006256
  Entrez Gene 19357 MouseEntrez Gene 314949 RatEntrez Gene 5885 Human
  UniProt ID O60216
• Synonyms: CDLS4; hHR21; HR21; HRAD21; MCD1; NXP1; SCC1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle