myosin light chain 1 (MYL1) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1H3]

CAT#: TA810680AM

MYL1 mouse monoclonal antibody,clone OTI1H3, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYL1 (RC202750, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYL1 (1:2000). Positive lysates LY409202 (100ug) and LC409202 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-MYL1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MYL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810680) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MYL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810680) (1:500)

Specifications

Product Data

• Clone Name: OTI1H3
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-150 of human MYL1 (NP_524146) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.5 kDa
• Gene Name: myosin light chain 1
• Database Link:
  NP_524146
  Entrez Gene 4632 Human
  UniProt ID P05976
• Synonyms: MLC1F; MLC3F