UPRT Mouse Monoclonal Antibody [Clone ID: OTI1D12]

CAT#: TA809847

UPRT mouse monoclonal antibody,clone OTI1D12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UPRT (RC205030, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UPRT (1:2000). Positive lysates LY408068 (100ug) and LC408068 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-UPRT monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-UPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809847) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809847) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-UPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809847) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809847) (1:1000)

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Specifications

Product Data

• Clone Name: OTI1D12
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human UPRT (NP_659489) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.6 kDa
• Gene Name: uracil phosphoribosyltransferase homolog
• Database Link:
  NP_659489
  Entrez Gene 139596 Human
  UniProt ID Q96BW1
• Background: This gene encodes uracil phosphoribosyltransferase, which catalyzes the conversion of uracil and 5-phosphoribosyl-1-R-diphosphate to uridine monophosphate (UMP). This reaction is an important part of nucleotide metabolism, specifically the pyrimidine salvage pathway. The enzyme localizes to the nucleus and cytoplasm. The protein is a potential target for rational design of drugs to treat parasitic infections and cancer. [provided by RefSeq, Nov 2009]
• Synonyms: FUR1; UPP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pyrimidine metabolism