Cyclophilin F (PPIF) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1G8]

CAT#: TA809070BM

PPIF mouse monoclonal antibody, clone OTI1G8 (formerly 1G8), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PPIF (Cat# RC223397, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPIF (Cat# TA809070)(1:2000). Positive lysates LY417109 (100ug) and LC417109 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-PPIF monoclonal antibody (1:200).

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PPIF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809070) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PPIF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809070) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PPIF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809070) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PPIF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809070) (1:150)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PPIF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809070) (1:150)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PPIF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809070) (1:150)

Show all 8 images

Specifications

Product Data

• Clone Name: OTI1G8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPIF(NP_005720) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.8 kDa
• Gene Name: peptidylprolyl isomerase F
• Database Link:
  NP_005720
  Entrez Gene 105675 MouseEntrez Gene 282819 RatEntrez Gene 10105 Human
  UniProt ID P30405
• Background: The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]
• Synonyms: Cyp-D; CyP-M; CYP3; CypD