MCEE Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1B5]

CAT#: TA808532BM

MCEE mouse monoclonal antibody, clone OTI1B5 (formerly 1B5), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MCEE (Cat# RC205018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCEE (Cat# TA808532)(1:2000). Positive lysates LY403176 (100ug) and LC403176 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808532) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808532) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808532) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808532) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808532) (1:150)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808532) (1:150)

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Specifications

Product Data

• Clone Name: OTI1B5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MCEE (NP_115990) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.6 kDa
• Gene Name: methylmalonyl-CoA epimerase
• Database Link:
  NP_115990
  Entrez Gene 84693 Human
  UniProt ID Q96PE7
• Synonyms: GLOD2

Reference Data

• Protein Pathways: Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation