LIME (LIME1) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI7A9]

CAT#: TA807654BM

LIME1 mouse monoclonal antibody, clone OTI7A9 (formerly 7A9), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIME1 (RC201909, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIME1 (1:2000). Positive lysates LY413516 (100ug) and LC413516 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI7A9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 30-295 of human LIME1(NP_060276) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.1 kDa
• Gene Name: Lck interacting transmembrane adaptor 1
• Database Link:
  NP_060276
  Entrez Gene 54923 Human
  UniProt ID Q9H400
• Background: LIME1 is a raft-associated transmembrane adaptor phosphoprotein that is preferentially expressed in hemopoietic cells, particularly T cells (Brdickova et al., 2003 [PubMed 14610046]; Hur et al., 2003 [PubMed 14610044]). [supplied by OMIM, Mar 2008]. ##Evidence-Data-START## Transcript exon combination :: AK000413.1, BC017016.1 [ECO:0000332] RNAseq introns :: single sample supports all introns ERS025081, ERS025083 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
• Synonyms: dJ583P15.4; LIME

Reference Data

• Protein Families: Transmembrane