MRPL15 Mouse Monoclonal Antibody [Clone ID: OTI10B10]

CAT#: TA807480

MRPL15 mouse monoclonal antibody,clone OTI10B10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MRPL15 (Cat# RC201471, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPL15 (Cat# TA807480)(1:500). Positive lysates LY415465 (100ug) and LC415465 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-MRPL15 monoclonal antibody. (HeLa:human;293T:human;K562:human; MCF7:human) (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MRPL15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807480) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-MRPL15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807480) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MRPL15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807480) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MRPL15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807480) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MRPL15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807480) (1:500)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MRPL15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807480) (1:500)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MRPL15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807480) (1:500)

Show all 9 images

Specifications

Product Data

• Clone Name: OTI10B10
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 28-296 of human MRPL15(NP_054894) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.2 kDa
• Gene Name: mitochondrial ribosomal protein L15
• Database Link:
  NP_054894
  Entrez Gene 27395 MouseEntrez Gene 29088 Human
  UniProt ID Q9P015
• Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q. [provided by RefSeq, Jul 2008]
• Synonyms: HSPC145; L15mt; MRP-L7; MRP-L15; RPML7