TEM1 (CD248) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1H6]

CAT#: TA807065BM

CD248 mouse monoclonal antibody,clone OTI1H6, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD248 (Cat# RC208576, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD248 (Cat# TA807065)(1:500). Positive lysates LY412436 (100ug) and LC412436 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CD248 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807065) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CD248 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807065) (1:150)

Specifications

Product Data

• Clone Name: OTI1H6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 20-300 of human CD248(NP_065137) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 80.7 kDa
• Gene Name: CD248 molecule
• Database Link:
  NP_065137
  Entrez Gene 57124 Human
  UniProt ID Q9HCU0
• Synonyms: CD164L1; TEM1

Reference Data

• Protein Families: Druggable Genome, Transmembrane