ADAMTS4 Mouse Monoclonal Antibody [Clone ID: OTI2D5]

CAT#: TA805005S

ADAMTS4 mouse monoclonal antibody, clone OTI2D5 (formerly 2D5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADAMTS4 (RC209226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADAMTS4. Positive lysates LY417514 (100ug) and LC417514 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2D5
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 296-577 of human ADAMTS4 (NP_005090) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 90 kDa
• Gene Name: ADAM metallopeptidase with thrombospondin type 1 motif 4
• Database Link:
  NP_005090
  Entrez Gene 66015 RatEntrez Gene 240913 MouseEntrez Gene 9507 Human
  UniProt ID O75173
• Background: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. [provided by RefSeq, Jul 2008]
• Synonyms: ADAMTS-2; ADAMTS-4; ADMP-1

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane