CD47 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: B6H12]

CAT#: TA804843BM

Purified CD47 mouse monoclonal antibody, clone B6H12, HRP conjugated

Product Images

Flow cytometric analysis of living 293T cells transfected with CD47 overexpression plasmid (RC218813), Red) using anti-CD47 antibody (TA804843). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

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  Flow cytometric Analysis of Jurkat live cells, using anti-CD47 antibody (TA804843), (Red), compared to a IgG1 isotype control (Black)

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  Flow cytometric Analysis of Sup-b15 live cells, using anti-CD47 antibody (TA804843), (Red), compared to a IgG1 isotype control (Black).

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  Flow cytometric analysis of living Raji cells, using anti-CD47 antibody (TA804843, Red), compared to an isotype control (green) (1:100).

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  Flow cytometric analysis of living Ramos cells, using anti-CD47 antibody (TA804843, Red), compared to an isotype control (green) (1:100).

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  Flow cytometric Analysis of living K562 cells, using anti-CD47 antibody (TA804843), (Red), compared to a mouse IgG control, (Blue) (1:100).

• Flow cytometric Analysis of living human peripheral blood cells, using anti-CD47 antibody (TA804843, a.right, b.red), compared to an IgG isotype control (a.left, b.blue) (1:100).

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Specifications

Product Data

• Clone Name: B6H12
• Applications: FC
• Recommended Dilution: FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Intact CD47 purified from placenta.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.4 kDa
• Gene Name: CD47 molecule
• Database Link:
  NP_001768
  Entrez Gene 961 Human
  UniProt ID Q08722
• Background: This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]
• Synonyms: IAP; MER6; OA3

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ECM-receptor interaction