TFF1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1F5]

CAT#: TA804519AM

TFF1 mouse monoclonal antibody,clone OTI1F5, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFF1 (RC207599, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFF1. Positive lysates LY418822 (100ug) and LC418822 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TFF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA804519) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TFF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA804519) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TFF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA804519) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-TFF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA804519) (1:500)

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Specifications

Product Data

• Clone Name: OTI1F5
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 25-84 of human TFF1 (NP_003216) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 9.15 kDa
• Gene Name: trefoil factor 1
• Database Link:
  NP_003216
  Entrez Gene 7031 Human
  UniProt ID P04155
• Background: Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]
• Synonyms: BCEI; D21S21; HP1.A; HPS2; pNR-2; pS2

Reference Data

• Protein Families: Druggable Genome, Secreted Protein