MSMB Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3D5]

CAT#: TA803510BM

MSMB mouse monoclonal antibody,clone 3D5, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSMB (RC202704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSMB. Positive lysates LY400872 (100ug) and LC400872 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-MSMB monoclonal antibody at 1:200.

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  Standard curve for ELISA analysis with MSMB recombinant protein (dilution range from 10pg/ml to 1ug/ml) using MSMB Capture Antibody (Cat# TA803512) at 5ug/ml and HRP conjugated MSMB Detection mAb (Cat# TA803510) at 0.05ug/ml.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MSMB mouse monoclonal antibody. (TA803510) Dilution: 1:150

Specifications

Product Data

• Clone Name: OTI3D5
• Applications: ELISA, IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified prostate specific antigen (MSMB) from human seminal fluid
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 10.7 kDa
• Gene Name: microseminoprotein, beta-
• Database Link:
  NP_002434
  Entrez Gene 4477 Human
  UniProt ID P08118
• Background: The protein encoded by this gene is a member of the immunoglobulin binding factor family. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues. The expression of the encoded protein is found to be decreased in prostate cancer. Two alternatively spliced transcript variants encoding different isoforms are described for this gene. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: HPC13; IGBF; MSP; MSPB; PN44; PRPS; PSP; PSP-94; PSP57; PSP94

Reference Data

• Protein Families: Secreted Protein, Transmembrane