DOT1L Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1D8]

CAT#: TA802482AM

DOT1L mouse monoclonal antibody, clone OTI1D8 (formerly 1D8), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L. Positive lysates LY410109 (100ug) and LC410109 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA802482)

• 

  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA802482)

Specifications

Product Data

• Clone Name: OTI1D8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-434 of human DOT1L (NP_115871) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: DOT1 like histone lysine methyltransferase
• Database Link:
  NP_115871
  Entrez Gene 84444 Human
  UniProt ID Q8TEK3
• Background: The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]
• Synonyms: DOT1; KMT4

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation